皱纹盘鲍性腺脂质成分分析  

Analysis of lipid component from Haliotis discus hannai ino gonad

作　　者：王竹清[1] 李八方[1] 李平林[1] 刘璐璐[1] 姚艳春[1] 赵雪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国海洋药物》2011年第3期37-42,共6页Chinese Journal of Marine Drugs

基　　金：国家支撑计划子课题(2008BAD94B07);国家自然科学基金(30800858)

摘　　要：目的皱纹盘鲍雄性、雌性性腺中富含的脂质成分进行对比研究。方法基于脂肪酸的存在形态,运用不同的化学衍生和GC/MS分析其脂肪酸组成和含量;用总磷脂钼蓝比色法测定其磷脂含量。结果在皱纹盘鲍雄性性腺中检测出脂肪酸47种,而在皱纹盘鲍雌性性腺中检测出41种,皱纹盘鲍雄性、雌性性腺中PU-FA主要是以AA和EPA为主,且含有一定量的单不饱和脂肪酸,其中雄性、雌性中C18∶1Δ9均在10%以上,不同的化学衍生法得到的结果有显著差异,表明其中脂肪酸的分子形态具有多样性;通过钼蓝比色法可知皱纹盘鲍性腺中磷脂含量较高,均在3%以上。结论皱纹盘鲍性腺具有再利用的价值,提高其附加值,增加经济收益。Objective To ivestigate the lipid components which are rich in the Haliotis discus hannai ino gonads andresearch their differences.Methods Based on the mode of fatty acid existence,the main fatty acids compositions and contents were analysed by GC/MS based on different chemical derivatization methods and the phospholipids contents were determined using molybdenum blue color concentrates.Results While 47 fatty acids were determined in the male Haliotis discus hannai ino gonad,41 fatty acids were found in the female Haliotis discus hannai ino gonad.And the majority of polyunsaturated fatty acids were arachidonic acid and eicosapentaenoic acid.There were also some monounsaturated fatty acids,in which the content of 9-octadecenic acid was above 10%.The results obtained by different chemical derivatization methods on fatty acids exist in significant differences.This showed the diversity of fatty acids＇ molecule forms.By molybdenum blue color concentrates,the contents of phospholipid were higher in the gonads（above 3%）.Conclusion The Haliotis discus hannai ino gonad has reusable value,and this could improve its added value and increase economic benefits.

关 键 词：皱纹盘鲍 性腺 脂质 脂肪酸 磷脂 

分 类 号：R916[医药卫生—药学]