CPG ODN对人胰腺癌细胞株Panc-1生物学行为的影响及TLR9蛋白在胰腺癌中的表达  

Influence of CPG ODN on the down-regulation of pancreas cell line PANC-1 and the expression of TLR9 in human pancreatic cancer

作　　者：吴汉青[1] 王博[1] 朱世凯[1] 张建军[1] 王春友[1] 吴河水[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《中华肝胆外科杂志》2011年第5期392-396,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金资助（30200272）

摘　　要：目的检测TLR9在人胰腺癌及胰腺癌细胞中的表达，研究CPG ODN2216对Panc-1细胞生物学行为的影响，并探讨其临床意义。方法通过免疫组织化学方法确认TI。R9蛋白在胰腺癌组织中的高表达，免疫荧光确认其在胰腺癌细胞株Panc-1中的高表达。通过细胞黏附、划痕实验、侵袭实验、细胞克隆及MTT增殖实验，研究CPG ODN2216对细胞黏附力、活动力及增殖力的影响。结果人胰腺癌标本及人胰腺癌细胞株Panc-1均高表达TLR9。划痕实验、体外黏附实验、基质胶侵袭实验、细胞克隆实验证明CPG ODN2216实验组细胞黏附力及活动力明显低于未加序列对照组。MTT法检测序列组增殖力明显低于未加序列对照组，且增殖活性具有时间剂量依赖性。结论TLR9基因与人类胰腺癌的侵袭转移潜能相关，外源配体CPG ODN2216的使用可明显抑制人类胰腺癌细胞Panc-1的侵袭、迁移能力。Objective To detect the expression of Toll-like receptor 9 （TLR9） in pancreatic cancer and to study the effect of CPG ODN2216 on the biological behavior of pancreatic cell carcinoma, and to explore their clinical significance. Methods Immunohistochemical method was used to examine the expression of TLR9 protein in pancreatic cancer tissue and immunofluorescence staining was also performed to detect TLR9 protein expression in pancreatic carcinoma cells. In vitro cell adhesion, wound-healing scrape assay, transwell invasion assay and cell colony formation assay were performed to assess the effect of CPG ODN2216 on the invasive properties of Panc-1 cells. Results TLR9 were highly expressed in the pancreatic cancer tissue and pancreatic carcinoma cells. In vitro experiments as cell spreading assays, cell adhesion, colony formation assay and invasion assays showed the cell adhesion and cell motility properties of CPG ODN 2216 group to be apparently weakened compared with the control group. MTT assay showed cell proliferation ability in the CPG ODN group to be notably decreased, and CPG ODN2216 had inhibitive effects on the growth of panc-1 ceils in a dose and time dependent manner. Conclusions TLR9 gene was correlated with the invasive and metastatic potentials of pancreatic carcinoma. The used of CPG ODN2216 induced the inhibition of migration and invasion of the Panc-1 cell line.

关 键 词：CPG ODN2216 胰腺肿瘤 TOLL样受体9 细胞生物学 

分 类 号：R735.9[医药卫生—肿瘤]