WT1反义寡核苷酸对卵巢癌细胞的抑制作用  

Inhibitory effect of antisense oligodeoxynucleotide targeting WT1 on ovarian carcinoma SKOV3 cells.

作　　者：霍晓溪[1] 任丽娜[2] 肖英[1] 卢翠敏[1] 孙莹[1] 尚丽新[1] 

机构地区：[1]北京军区总医院妇产科,北京100700 [2]中国医科大学附属第一医院产科,沈阳110003

出　　处：《中国优生与遗传杂志》2011年第6期22-24,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的观察WT1反义寡核苷酸对人卵巢癌细胞株SKOV3的抑制作用。方法以人卵巢癌细胞株SKOV3为研究对象,应用ASODN技术,将WT1-ASODN转染到SKOV3细胞中,利用MTT法检测WT1寡核苷酸的最适作用浓度和作用时间及WT1反义寡核苷酸对SKOV3细胞的杀伤活性,流式细胞仪检测分析WT1反义寡核苷酸对SKOV3细胞周期和细胞凋亡的影响。结果脂质体介导的WT1反义寡核苷酸在体外能够明显抑制卵巢癌SKOV3细胞增殖,诱导细胞凋亡。结论 WT1反义寡核苷酸能诱导卵巢癌细胞凋亡,从而抑制卵巢癌细胞的增殖,可望成为一种卵巢癌临床有效的治疗方法。Objectives：To study the inhibitory effect of WT1 antisense oligodeoxynucleotides（ASODN） transfection on Ovarian Carcinoma SKOV3 cells.Methods： Ovarian Carcinoma cell SKOV3 lines were cultured with WT1-ASODN in a wide range of concentration for various time.Cell activity and the effect of WT1 ASODN on cell proliferation were assayed by MTT.The changes of cell cycle and cell apoptosis were analyzed by FCM.Results： The growth of the ovarian cancer cell lines SKOV3 transfected by WT1 ASODN became significantly slow and its activity reduced.WT1 antisense phosphorothioate oligonucleotides not only inhibited the proliferation but also induce the apoptosis in SKOV3 ovarian carcinoma cells.Conclusion： WT1 antisense phosphorothioate oligonucleotides could not only induce the apoptosis but also inhibite the proliferation in SKOV3 ovarin carcinoma cell line.Antisense oligonucleotides of WT1 may become a kind of treatment for ovarian carcinoma.

关 键 词：WT1基因 上皮性卵巢癌 反义寡核苷酸 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]