脂肪酶选择性水解酰胺工艺条件的初步研究  

Investigation of the Process for Lipase-Catalyzed Enantioselective Hydrolysis of Amides

作　　者：曾玲[1] 胡蓉[1] 应向贤[1] 章银军[1] 汪钊[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《化学反应工程与工艺》2011年第1期39-43,共5页Chemical Reaction Engineering and Technology

摘　　要：对脂肪酶Nov435选择性水解拆分2-氯-N-(1-(4-氯苯基)乙基)乙酰胺的工艺进行了研究。通过对反应介质、转化时间、底物浓度、温度和转速等影响因素的考察,得到较优的拆分工艺为:反应介质为含水体积分率为0.2%的甲苯,酶添加量0.04g/mL,底物浓度为0.3%,反应温度40℃,转速为220r/min,转化时间为24h。在该拆分工艺条件下,产物2-氯-N-(1-(4-氯苯基)乙基)乙酰胺最高得率34.3%,产物的对映体过量值为99%以上。The process of enantioselective hydrolysis of 2-chloro-N- （,l-（4-CnlOropnenyl）ethyl acetamide was investigated using the lipase Novozym435 as a biocatalyst. The effects of various factors on the hydrolysis reaction were examined, including reaction medium, reaction time, temperature and shaking rate. The enzymatic hydrolysis conditions were optimized as following： toluene containing 0.002 mL-water/mL as solvent, enzyme concentration 0.04 g/mL, substrate concentration 0.3%, temperature 40℃, shaking rate 220 r/min, reaction time 24 h. The enantiomeric excess against 4-cholo-phenyl ethylamine was more than 99% and its yield was as high as 34.3% under the optimal conditions mentioned above.

关 键 词：2-氯-N-(1-(4-氯苯基)乙基)乙酰胺 脂肪酶 Novozym435 水解反应 对映体 手性拆分 

分 类 号：TQ033[化学工程]