抗癌生物活性肽对人胃癌BGC-823细胞周期相关蛋白CDC2、CDK4表达的影响  

THE EFFECT OF ANTI-CANCER BIOACTIVE PEPTIDE ON THE EXPRESSION OF CDC2 AND CDK4 OF HUMAN GASTRIC CANCER CELLS

作　　者：温再和[1] 崔宏伟[2] 张嘉玲[2] 苏秀兰[2] 

机构地区：[1]内蒙古医学院附属医院麻醉科,内蒙古呼和浩特010050 [2]内蒙古医学院附属医院临床医学研究中心

出　　处：《内蒙古医学院学报》2011年第2期89-92,共4页Acta Academiae Medicinae Neimongol

基　　金：国家自然科学基金(30860327)

摘　　要：目的:探讨抗癌生物活性肽对人胃癌细胞周期相关蛋白CDC2、CDK4的影响及其作用机制。方法:实验分为活性肽组及对照组,将不同浓度的抗癌生物活性肽按不同时间作用于培养的人胃癌细胞株(BGC-823),应用MTT法检测增殖抑制率。建立荷胃癌裸鼠模型,给予生理盐水作为阴性对照组,应用免疫组化技术对抗癌生物活性肽作用前后的胃癌裸鼠肿瘤中CDK4、CDC2的表达进行检测。结果:不同浓度的抗癌生物活性肽对胃癌细胞均有增殖抑制作用;抗癌活性肽S-2作用后人胃癌组织中的CDK4表达明显低于对照组(P<0.01),而CDC2表达高于对照组(P<0.01)。结论:抗癌生物活性肽具有抗胃癌细胞增殖作用,其抗肿瘤作用机制可能是通过下调CDK4和上调CDC2的表达实现。Objective：To study the effect of anti-cancer bioactive peptide（ACBP） on the expression of CDC2 and CDK4 of human gastric cancer cells and the possible anti-cancer mechanism.Methods：Human gastric cancer BGC-823 cells were cultured in vitro and gastric cancer loaded nude mice mode were established.Test groups were given ACBP and control groups were given NS.Then immunohistochemistry method was carried out to detect the expression of CDC2 and CDK4.Result：Different concentrations of anti cancer bioactive peptide had the function of inhibiting the proliferation of BGC-823 cells.The expression of CDK4 of test groups were significant lower than those of control groups（P0.01）;but the expression of CDC2 of test groups were significantly higher than those of control groups（P0.01）.Conclusion：We have confirmed that ACBP execute the function of anti-tumor by deregulating CDK4 and up regulating CDC-2.

关 键 词：胃癌 抗癌生物活性肽 CDC2 CDK4 

分 类 号：R735.2[医药卫生—肿瘤]