植物乳杆菌NCU116在模拟人体消化环境中的耐受力  

Tolerance of Lactobacillus plantarum NCU116 in Stimulated Digestive Environments

作　　者：熊涛[1] 宋苏华[1] 黄锦卿[1] 黄寅[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家贡点实验室,江西南昌330047

出　　处：《食品科学》2011年第11期114-117,共4页Food Science

基　　金：国家自然科学基金项目(31060224);教育部留学回国人员创业基金项目(赣教财字[2009]135号)

摘　　要：模拟人体消化道环境,即在人工胃液、人工肠液、胆盐和高盐环境下对植物乳杆菌NCU116的耐受力进行研究。结果表明:植物乳杆菌NCU116在pH1.5和pH2.5的人工胃液中培养3h,存活率分别达到了32.62%和45.76%,在pH3.5以上的人工胃液中能保持很高的存活率;在人工肠液中作用4h,存活率达到了49.63%;牛胆盐环境中培养24h后的NCU116活菌数随牛胆盐质量浓度(0.03～1.00g/mL)的增加而降低,但活菌数均保持在107CFU/mL以上;NaCl高盐环境中培养24h后的活菌数随NaCl质量浓度(1～8g/100mL)的增加而稍有降低,当NaCl质量浓度为8g/100mL时,活菌数仍在108CFU/mL以上。这表明植物乳杆菌NCU116对人工胃液、人工肠液、胆盐和高盐具有较好的耐受力,在食品和保健品工业中具有良好的应用前景。The tolerance of Lactobacillus plantarum NCU116 in several stimulated human digestive environments such as artificial gastric fluid,artificial intestinal fluid,bile salt environment and high salt environment was investigated.The results showed that survival rates of Lactobacillus plantarum NCU116 were 32.62% and 45.76% after 3 h of incubation in artificial gastric fluid with pH 1.5 and pH 2.5,respectively,and a higher survival rate was achieved when the pH of artificial gastric fluid equaled or exceeded 3.5.After 4 h incubation in artificial intestinal fluid,the strain revealed a survival rate of 49.63%.The number of viable NCU116 cells after 24 hours of incubation in bile salt environment exhibited a downward trend as the concentration of bile salt increased from 0.03 to 1.00 g/100 mL,and remained higher than 107 CFU/mL at the highest bile salt concentration.The number of viable NCU116 cells after 24 hours of incubation in high-concentration NaCl environment slightly declined with increasing NaCl concentration from 1 to 8 g/100 mL,and was higher than 108 CFU/mL when the NaCl concentration was 8 g/100 mL.In summary,Lactobacillus plantarum NCU116 has excellent tolerance in artificial gastric fluid,artificial intestinal fluid,bile salt environment and high salt environment and will thus have promising application prospects in the food and healthcare food industries.

关 键 词：植物乳杆菌NCU116 胃肠道环境 高盐环境 耐受性 

分 类 号：TS201.3[轻工技术与工程—食品科学]