两种灵芝多糖组分单糖组成分析及其对5-氟尿嘧啶抑制小鼠肿瘤的影响  

Monosaccharide Composition Analysis of Two Polysaccharide Fractions from Ganoderma lucidum and Their Effects on inhibiting Tumor Growth in Mice by 5-Fluracil

作　　者：刘艳芳[1] 唐庆九[1] 张劲松[1] 杨焱[1] 周帅[1] 吴迪[1] 贾薇[1] 冯娜[1] 

机构地区：[1]国家食用菌工程技术研究中心,农业部应用真菌资源与利用熏点开放实验室,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201106

出　　处：《食品科学》2011年第11期288-291,共4页Food Science

基　　金：国家“863”计划项目(2007AA10Z341);上海市农业科学院院发基金项目(农科发2008-04)

摘　　要：目的:分析比较两种灵芝多糖组分的单糖组成,并考察其对5-氟尿嘧啶(5-FU)抑制小鼠肿瘤的影响。方法:采用DEAE离子柱层析和离子交换型大孔树脂结合超滤,制备得到两种灵芝多糖组分BW和GLB。对两个样品的糖含量、单糖组成及比例进行比较分析;并将两种多糖组分与5-FU联合使用治疗小鼠肿瘤,比较抑瘤率和脾脏指数等免疫指标的变化。结果:BW和GLB两个组分糖含量接近,分别为54.28%和51.64%;单糖组成相同,主要都由岩藻糖、半乳糖、葡萄糖和甘露糖组成;单糖比例有差异,GLB所含的葡萄糖和甘露糖的比例高于BW。动物实验结果表明,BW与5-FU合用可明显增强5-FU对小鼠肿瘤的抑制率,GLB对5-FU引起的小鼠骨髓有核细胞数减少有明显的拮抗作用。结论:两种灵芝多糖组分对5-FU抑制小鼠肿瘤的影响不同,BW表现出明显的增效作用,GLB具有减轻5-FU骨髓抑制副作用的效果。Objective： To analyze the monosaccharide composition of two polysaccharide fractions from Ganoderma lucidum,and then explore their effects on inhibition of tumor growth in mice by 5-fluracil（5-FU）.Methods： Polysaccharide fractions named as BW and GLB were separated from the fruit bodies of Ganoderma lucidum by DEAE-Sepharose Fast Flow column,macroporous resin adsorption and ultrafiltration.The monosaccharide composition of both polysaccharide fractions was comparatively analyzed by high performance anion exchange chromatography.Tumor-bearing mice were treated with BW or GLB coupled with 5-FU.Tumor inhibition rate and immune index were determined.Results： The polysaccharide contents of BW and GLB were 54.28% and 51.64%,respectively.The major monosaccharide components in both extracts were fucose,galactose,glucose and mannose.The contents of glucose and mannose in GLB were much higher than those in BW.Meanwhile,the combinatorial treatment for tumor in mice using BW and 5-FU could obviously improve the inhibition rate for tumor growth compared with the single treatment of 5-FU.However,GLB could antagonize 5-FU-induced bone marrow cell reduction in mice.Conclusion： BW combined with 5-FU could improve the anti-tumor effect of 5-FU,and GLB combined with 5-FU could decrease the toxicity of 5-FU on bone marrow cells.

关 键 词：灵芝 多糖 单糖组成 抑制肿瘤 

分 类 号：R96[医药卫生—药理学] TS201.2[医药卫生—药学]