复合溶剂选择性纯化紫杉醇的工艺研究  

Selective Purification Study of Paclitaxel with Complex Solvents

作　　者：甘勇军[1] 廖家荣[2] 严明志 张倩[2] 高宏伟 胡湘南[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆市400016 [2]四川遂宁市中医院药剂科,遂宁市629000 [3]重庆美联制药有限公司,重庆市401122

出　　处：《中国药房》2011年第23期2145-2147,共3页China Pharmacy

摘　　要：目的:研究复合溶剂选择性纯化紫杉醇的方法和工艺条件。方法:采用不同种类的溶剂在不同条件下对紫杉醇进行精制,以高效液相色谱法测定的紫杉醇粗品含量和主要杂质峰峰面积为指标,考察最佳的溶剂种类和工艺参数。结果:最佳工艺为纯化强极性杂质较高的紫杉醇,采用紫杉醇-丙酮-乙酸乙酯-正己烷(1g∶8mL∶6mL∶7mL)于-25℃冷冻24 h;纯化弱极性杂质较高的紫杉醇,采用紫杉醇-丙酮-三氯甲烷-正己烷(1g∶8mL∶6mL∶7mL)于-25℃冷冻24 h。结论:采用丙酮、正己烷分别与乙酸乙酯或三氯甲烷纯化紫杉醇,对于不同极性的杂质具有良好选择性,可用于紫杉醇的选择性纯化。OBJECTIVE：To study the selective purification study of paclitaxel with complex solvents.METHODS：The paclitaxel was refined using different types of solvents under different conditions.The best solvent types and process parameters were obtained from HPLC detection of paclitaxel content and the main impurity peak area as index.RESULTS：The optimal technology was to purify strongly polar paclitaxel with high impurity,paclitaxel-acetone-ethyl acetate-n-hexane（1 g：8 mL：6 mL：7 mL） were frozen at-25 ℃for 24 h;to purify weak polar paclitaxel with high impurity,paclitaxel-acetone-chloroform-n-hexane（1 g：8 mL：6 mL：7 mL） were frozen at-25 ℃ for 24 h.CONCLUSION：The uses of acetone or n-hexane and ethyl acetate or chloroform were refined for different polar impurities with good selectivity,and it can be used for the selective purification of paclitaxel.

关 键 词：紫杉醇 复合溶剂 纯化 

分 类 号：R284.2[医药卫生—中药学] R943[医药卫生—中医学]