白藜芦醇对TRAIL诱导乳腺癌MDA-MB-231细胞凋亡的影响  

Effects of Resveratrol on TRAIL-induced apoptosis of MDA-MB-231 breast cancer cells

作　　者：李岩[1] 赵丹[1] 陈洋[1] 刘新莉[1] 马萍[1] 

机构地区：[1]中国医科大学附属一院肿瘤研究所二室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2011年第14期1572-1576,共5页China Journal of Modern Medicine

摘　　要：目的研究白藜芦醇对TRAIL诱导乳腺癌MDA-MB-231细胞增殖及凋亡的影响。方法 MTT法检测细胞增殖;AnnexinⅤ染色并流式细胞术检测细胞凋亡;分光光度法检测Caspase-8、Caspase-3相对活性;DAPI细胞核染色法观察细胞凋亡。结果 50μmol/L和100μmol/L白藜芦醇分别与50 ng/mL TRAIL联合作用后,对MDA-MB-231细胞增殖的抑制率为(62.43±2.07)%和(87.32±1.44)%;而细胞凋亡率分别为(53.59±1.44)%和(73.72±1.96)%,与单独应用相应浓度的白藜芦醇和TRAIL比较,差异均存在显著性(P<0.01)。50 ng/mL TRAIL分别与50μmol/L和100μmol/L白藜芦醇联合作用后,Caspase-8、Caspase-3的相对活性为(0.282±0.008)、(0.386±0.013)及(0.437±0.05)、(0.521±0.03),与对照组及单用TRAIL、白藜芦醇比较均明显增强,差异显著(P<0.01)。结论 TRAIL与白藜芦醇联合可协同抑制MDA-MB-231细胞增殖并诱导其凋亡,白藜芦醇增强TRAIL诱导细胞凋亡的作用可能是通过促进Caspase-8和Caspase-3的表达来实现。【Objective】 To explore the effects of Resveratrol on TRAIL-induced apoptosis of MDA-MB-231 breast cancer cell line.【Methods】 The proliferation was detected by MTT assay.Apoptosis of cells was analyzed by Annexin V staining combined with flow cytometry assay.The relative activity of Caspase-8 and Caspase-3 were examined by spectrophotometric method.Nuclear was stained with DAPI and its morphology was observed.【Results】 Exposure of cell to 50 ng/mL TRAIL combined with 50 μmol/L and 100 μmol/L Resveratrol for 12 h increased inhibitory effect on cell proliferation which were 62.43±2.07%,87.32±1.44% respectively,as well as enhanced the apoptotic percentage which were 53.59±1.44% and 73.72±1.96% separately,compared with that of the cells incubated with TRAIL or Resveratrol alone.The activity of Caspase-8 and Caspase-3 in the cells treated with TRAIL combined with Resveratrol was also much higher than that of cells treated with TRAIL or Resveratrol alone.【Conclusion】 Resveratrol could enhance the effect of TRAIL on proliferation inhibitory and apoptosis promotion of breast cancer cells via increase the expression of Caspase-8 and Caspase-3.

关 键 词：乳腺癌 凋亡 肿瘤坏死因子相关凋亡诱导配体 白藜芦醇 CASPASE 

分 类 号：R737.9[医药卫生—肿瘤]