产紫红色素菌株的筛选及诱变  

Screening and mutation of amaranth pigment-producing fungus

作　　者：胡博涵[1] 刘素纯[2,3] 何理[4] 李海飞[1] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128 [2]湖南省发酵食品工程技术研究中心,湖南长沙410128 [3]食品科学与生物技术湖南省重点实验室,湖南长沙410128 [4]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品与机械》2011年第3期29-31,共3页Food and Machinery

基　　金：2010年湖南省大学生研究性学习和创新性实验计划项目(编号:SCX1010)

摘　　要：以土壤和3种水果为样品,马铃薯-抗生素培养基为基质,采用稀释平板法分离产紫红色素的菌株,并以产紫红色素菌株为出发菌株进行紫外诱变。结果表明:从苹果表面上获得1株产紫红色素的真菌,通过个体形态和菌落特征初步分析为镰孢菌属菌株(fusarium sp.),该菌以马铃薯-蔗糖(35%)为基质发酵,获得胞内紫红色素OD值为0.790,胞外色素OD值为0.538,该突变菌株获得其胞内紫红色素OD值为0.895,胞外色素OD值为0.503,且该突变菌株产生色素的速度要快于出发菌株1d。Dilution plate method was adopted to screen amaranth pigment-producing fungus from soil and fruits using potato-antibiotic culture medium.The result indicated that a fungus strain obtained from apple surface with amaranth pigment-producing activity was identified as Fusarium oxysporum by observation of its cell shape and colony characteristics.After fermentation of the original screened strain in potato sucrose-（35%） nutrient medium,the OD values of intracellular and extracellular amaranth pigment were 0.790 and 0.538,respectively.As for the mutation-treated strain,the OD values of intracellular and extracellular amaranth pigment were 0.895 and 0.503.Besides,the mutation-treated strain could finish pigment production one day earlier than the original strain.Our work laid a foundation for the research on production of amaranth pigment from microorganism fermentation.

关 键 词：紫红色素 OD值 诱变 

分 类 号：TS202.3[轻工技术与工程—食品科学]