丛梗孢酵母发酵产赤藓糖醇的响应面优化  

Optimization of Erythritol Production by Moniliella acetoabutans Using Response Surface Methodology

作　　者：刘鹏[1] 王泽南[1] 张仕发[1] 李莹[1] 

机构地区：[1]合肥工业大学生物与食品工程学院合肥230009

出　　处：《中国生物工程杂志》2011年第5期69-74,共6页China Biotechnology

基　　金：安徽省教育厅重点资助项目(Kj2010A27)

摘　　要：为了提高丛梗孢酵母发酵产赤藓糖醇的产量,在前期单因素实验结果的基础上,利用Plackett-Burman实验设计对影响其产赤藓糖醇的发酵条件进行评估并筛选出了影响显著的3个因素:葡萄糖、初始pH和温度。采用响应面法进行实验方案设计,利用SAS软件对其结果进行二次回归分析,确定了优化后的发酵条件为:葡萄糖260g/L、酵母膏4g/L、KH2PO4 0.2g/L、MnSO4.4H2O 0.05g/L、CuSO4.5H2O 0.04g/L、初始pH4.2、温度31℃、装液量20%、转速150r/min、接种量4%。在该条件下发酵90h,发酵液中的赤藓糖醇含量,较优化前提高10.8%达到75.63g/L,转化率提高9.4%为36.12%。The culture medium and fermentation conditions of erythritol production by Moniliella acetoabutans was optimized by response surface methodology.According to the results of mono-factors experiments,a Plackett-Burman design was used to investigate and evaluate the influence of related factors,three factors are statistically significant： glucose concentration,initial pH value and temperature.Box-Behnken was employed to design.The quadratic regression analysis was conducted on the optimized results by using SAS software,it was found that the optimum conditions for erythritol production were glucose26%,yeast extract 0.4%,KH2PO4 0.2g/L,MnSO4·4H2O 0.05g/L,CuSO4·5H2O 0.04g/L,initial pH value 4.2,temperature 31℃,broth content 20%,shaking speed 150r/min,inoculum 4%.Under this condition,the concentration of erythritol reached to 75.63g/L in the shake-flask experiments after 90h fermentation,increased by 10.8% than before,the yield was 36.12%,increased by 9.4% than before.

关 键 词：丛梗孢酵母 赤藓糖醇 PLACKETT-BURMAN设计 响应面法 

分 类 号：Q939.97[生物学—微生物学]