整合型HBV-DNA前C区基因突变与肝细胞癌的相关性  

THE CORRELATION BETWEEN GENE MUTATION ON PRECORE REGION OF HBV DNA INTEGRATION AND HEPATOCELLULAR CARCINOMA

作　　者：王修海[1,2,3] 王培林[1,2,3] 张杰 邱法波[1,2,3] 王兰 

机构地区：[1]青岛医学院生物学教研室 [2]青岛医学院附属医院普外科 [3]青岛市市北区医院五官科

出　　处：《青岛医学院学报》1999年第2期79-80,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金

摘　　要：目的探讨整合型ＨＢＶ－ＤＮＡ前Ｃ区基因突变与肝细胞癌的关系。②方法应用聚合酶链反应－单链构象多态技术，对２９例肝癌、１２例慢性肝硬化和１４例非肝病病人的肝组织内整合型ＨＢＶ－ＤＮＡ前Ｃ区进行突变分析。③结果肝癌、肝硬化和正常肝组织中整合型ＨＢＶ－ＤＮＡ阳性率分别为９６．５５％，６６．６７％和１４．２９％，组间比较差异有显著性（χ２＝２９．４３，Ｐ＜０．００１），３组整合型ＨＢＶ－ＤＮＡ前Ｃ区突变率分别为４１．６％，２５．０％和０，组间比较差异有显著性（χ２＝１２．４４，Ｐ＜０．０５）。④结论肝组织中整合型ＨＢＶ－ＤＮＡ前Ｃ区突变与肝细胞癌的发生和发展相关。Objective\ To explore the relationship between the gene mutation on precore region of HBV DNA integration and the hepatocellular carcinoma.\ Methods\ The gene mutation on precore region of HBV DNA integration in liver tissues of 29 cases with hepatocellular carcinoma, 12 cases with liver cirrhosis and 14 cases with non liver diseases were detected with PCR SSCP technique.\ Results\ The positive rates of HBV DNA integration among tumor liver tissues, cirrhotic liver tissues and normal liver tissues were 96.55%, 66.67% and 14.29%, respectively. The differences between the three groups were more significant( χ 2=29.43, P <0.001). The gene mutation rates on precore region of HBV DNA integration in three groups were 41.6%, 25.0 % and 0, respectively. The differences between the three groups were significant( χ 2=12.44, P< 0.05).\ Conclusion\ The gene mutation on precore region of HBV DNA integration in liver tissues was associated with the morigenesis and development of hepatocellular carcinoma.

关 键 词：肝细胞癌 乙型肝炎病毒 HBV-DNA 基因突变 

分 类 号：R735.702[医药卫生—肿瘤]