猪链球菌2型荧光PCR检测方法与常规检测方法的比较  

Comparison of Detection of Streptococcus suis Serotype 2 by Real-time PCR and Conventional Detection Methods

作　　者：宋立[1] 高志强[2] 杨承槐[1] 宁宜宝[1] 张鹤晓[2] 吴丹[2] 张利峰[2] 刘洋[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]北京出入境检验检疫局,北京100026

出　　处：《中国兽药杂志》2011年第6期8-10,19,共4页Chinese Journal of Veterinary Drug

摘　　要：为验证猪链球菌2型荧光PCR检测方法对临床样品检测的敏感性和适用性以及该方法所拥有的独特优点,分别用荧光PCR法、常规PCR法和细菌分离法对人工感染猪链球菌2型的小鼠肝脏和发病猪的心、肝、脾、肾等实质器官和血液、喉拭子进行抗原检测。结果显示,荧光PCR法检出率为70.8%,明显高于普通PCR法(检出率为20.8%),也高于常规细菌分离法(检出率为45.8%)。由于临床样品常常会被其他细菌污染,细菌分离法很难准确分离到链球菌。但荧光PCR法不受其他细菌污染的影响,对实验室培养的猪链球菌2型菌液,该方法检测滴度可达10-6/0.1mL(42～52 CFU/0.1 mL),而普通PCR方法检测滴度仅为10-4。In order to validate the clinical sensitivity and applicability of real-time PCR assay for detection of Streptococcus suis serotype 2,rat liver of artificial infection,parenchymal organs（e.g.heart,liver,spleen,kidney and so on）as well as blood and throat swabs of swine which were challenged Streptococcus suis serotype Ⅱ was performed by comparison with conventional PCR method and bacterial isolation method.The results indicated that detection rate of real-time PCR assay was 70.8% which was obviously higher than that of conventional PCR method（20.8%） and bacterial isolation method（45.8%）.Meanwhile,clinical samples were generally contaminated by other bacterium,and could be hardly isolated specific to Streptococcus accurately,whereas this assay was not interfered with bacterial contamination.For liquid cultures of Streptococcus suis serotype Ⅱ in laboratory,detected titre of real-time PCR was 10-6/0.1 mL（42~52 CFU/0.1 mL）,while that of conventional PCR method was just 10-4.

关 键 词：猪链球菌2型 荧光PCR检测 检测方法比较 

分 类 号：S852.611[农业科学—基础兽医学]