Construction of Mutation Library in Haemophilus parasuis by Inserting Tn5 Transposon and the Screening of Attenuated Strain  

副猪嗜血杆菌EZ-Tn5转座子插入突变体库的构建及减毒株的筛选(英文)

作　　者：贺云霞[1] 徐慧[1] 叶飞[1,2] 孙慧玲[1] 王宏俊[1] 龚玉梅[1] 张莉[1] 黄秀芬[1] 张培君[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]首都师范大学生命科学学院,北京100048

出　　处：《Agricultural Science & Technology》2011年第2期295-300,共6页农业科学与技术（英文版）

基　　金：Supported by National High Technology Research and Development Program of China(2006AA10A206);National Natural Science Foundation of China(31001072);the Fund of Beijing Academy of Agriculture and Forestry Sciences for Young Scholars(QNJJ201012)~~

摘　　要：[Objective] The study aimed to obtain attenuated strain of Haemophilus parasuis.[Method] Tn5 transposon technology was used to construct a library of mutants.Positive mutants were screened by kanamycin resistance.False positive was identified by PCR and then removed.Mice were infected to detect the virulence of mutants.The bionomics of attenuated strains were detected,too.[Result] The attenuated mutants showed similar reproductive activity to that of wild strain.The virulence of mutants was still stable after 30 passages.[Conclusion] This study provided foundation for exploring the virulence factors and pathogenic mechanism of HPS.[目的]获得副猪嗜血杆菌减毒株.[方法]应用转座子技术构建转座子插入突变体库,卡那抗性筛选阳性菌株,PCR扩增卡那特异片段去除假阳性,小鼠感染试验检测突变株毒力,并对获得的减毒株进行生物学特性检测。[结果]所获得减毒突变菌株具有与野毒株相似的增殖能力,传代后毒力稳定,遗传学特性稳定。[结论]该研究结果为进一步探讨HPS毒力因子、致病机制奠定了基础。

关 键 词：Haemophilus parasuis TRANSPOSON Mutation library Attenuated strains 

分 类 号：S852.61[农业科学—基础兽医学]