LSA-7树脂分离纯化爬山虎果实红色素工艺优化  

Optimization of Purification Process for Red Pigment from Parthenocissus tricuspidate Fruits by LSA-7 Resin Adsorption

作　　者：卜晓英[1] 董爱文[1] 陈晓华 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]张家界市环境保护局,湖南张家界427000

出　　处：《食品科学》2011年第12期111-114,共4页Food Science

基　　金：湖南省科技厅计划项目(2008TP4008-32005JT1075);湖南省教育厅科技项目(05C139);湖南省高校"林产资源化学与林化产品开发"科技创新团队支持计划项目

摘　　要：对常用的10种大孔吸附树脂应用于爬山虎熟果红色素分离效果考察,其中LSA-7型树脂具有最佳分离效果。进一步优化LSA-7树脂动态吸附分离红色素的条件和应用紫外分光光度法和高效液相色谱法对分离纯化得到的红色素进行品质检测。结果表明:在温度30℃、进样质量浓度30mg/mL时流经LSA-7吸附柱的红色素溶液体积达到两倍床层体积,树脂吸附接近饱和;当洗脱剂以3BV/h通过吸附床时,洗脱下来的红色素纯度大于96.7%,所含色素成分与薄层硅胶H分离的该色素成分一致,因此LSA-7树脂完全适合工业化动态生产爬山虎熟果红色素。Ten kinds of commonly used macroporous resin were compared for their effectiveness in purifying crude red pigment obtained from Parthenocissus tricuspidate fruits by fractional solvent extraction.It was found that LSA-7 resin had the best purification performance.Further,operating conditions for purifying red pigment from Parthenocissus tricuspidate fruits by dynamic adsorption onto LSA-7 resin column were optimized and UV spectrophotometry and HPLC were used to analyze red pigment samples.The adsorption of LSA-7 resin almost reached its saturation under the following conditions： temperature of 30 ℃,sample concentration of 30 mg/mL and sample loading amount of 2 BV.Elution at a flow rate of 3 BV/h resulted in a red pigment purity of more than 96.7%.Moreover,the resulting pigment composition was the same as that obtained by thin layer silica gel H separation.Therefore,LSA-7 resin is suitable for industrial dynamic production of red pigment from Parthenocissus tricuspidate fruits.

关 键 词：爬山虎 红色素 LSA-7树脂 动态吸附 

分 类 号：O652.63[理学—分析化学] R284.2[理学—化学]