链霉菌碱性脂肪酶的研究  

STUDIES ON ALKALINE LIPASE BY STREPTOMYCES

作　　者：周晓云[1] 黄建宁[2] 王慧中[3] 王荣伟 

机构地区：[1]浙江工业大学生物工程系,杭州310014 [2]浙江省农科院微生物研究所,杭州310021 [3]杭州师范学院生物系,杭州310012

出　　处：《应用与环境生物学报》1999年第5期529-532,共4页Chinese Journal of Applied and Environmental Biology

基　　金：浙江省科委重点项目;浙江省自然科学基金

摘　　要：从杭州土壤中获得的一支链霉菌，通过ＵＶ、ＤＥＳ和Ｃｏ６０ － γ射线的５ 代诱变，选育成功了高产的Ｚ９４－ ２ 菌株，经过对Ｚ９４ －２ 的发酵研究，产碱性脂肪酶活力达５９６ ｕｍＬ，其最适培养基（λｕ Ｌ－１） 为：糊精１０、黄豆饼粉３０、尿素１０ 、Ｋ２ＨＰＯ４０ ．５、ＭｇＳＯ４·７Ｈ２Ｏ０ ．５、ＮａＣｌ１ 和ＡＥＯ９ ０．５ ，产酶的最适条件为：初始ｐＨ９．０～１０．０ ，２６℃培养４８ ｈ．对Ｚ９４－ ２Streptomyces Z94-2, a alkaline lipase producing strain, was obtained through mutation for 5 generations by UV, DES and Co 60 - γ from a wild strain in the soil in Hangzhou. The optimal medium for the strain ( ρ /g L -1 ): soybean meal 30, urea 10, dextrin 10, KH 2PO 4 0.5, MgSO 4·7H 2O 0.5, NaCl 1 and AEO 9 (nonionic surfactant) 1, and the optimal conditions for lipase production: initial pH 9.0～10.0, culture temperature 26℃ and culture time 48 h. The maximal lipase activity was 596 (u/mL).The enzyme characteristics of Streptomyces Z94-2 were compared with those of other strains.

关 键 词：碱性脂肪酶 链霉菌 产酶条件 菌株 筛选 

分 类 号：Q939.132[生物学—微生物学] TQ925.6[轻工技术与工程—发酵工程]