银杏抗氧化肽的分离纯化及活性鉴定  

Purification and Activity Identification of Ginkgo Antioxidant Peptide

作　　者：贾韶千[1] 吴彩娥[1] 范龚健[1] 李婷婷[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037

出　　处：《农业机械学报》2011年第6期152-155,共4页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金资助项目(30872055);高等学校博士点专项科研基金资助项目(200802980004);江苏省普通高校研究生科研创新计划资助项目(CX10B_244Z)

摘　　要：为了分离制备银杏抗氧化肽,通过葡聚糖凝胶Sephadex G-25、Sephadex G-10和半制备型液相色谱对银杏抗氧化肽进行纯化,得到了具有抗氧化活性的肽段。纯化后的银杏抗氧化肽经过高效液相色谱鉴定为单一组分,采用液相色谱串联四极杆飞行时间质谱测定其相对分子量为452.21 Da。抗氧化试验结果表明,纯化后的银杏抗氧化肽质量浓度为1.0 mg/mL时,对羟自由基和DPPH自由基具有很强的清除作用,清除率分别达到80.26%和83.57%。In order to separate and prepare ginkgo antioxidant peptide,Sephadex G-25 gel filtration chromatography,Sephadex G-10 gel filtration chromatography and reverse phase-high performance liquid chromatography（RP-HPLC） were used to purify ginkgo peptide.The purity of the ginkgo peptide was confirmed by HPLC.Liquid chromatography quadrupole time of flight mass showed that the relative molecular mass was 452.21 Da.Results of antioxidation test indicated that ginkgo antioxidant peptides exhibited strong scavenging capability to hydroxyl radicals and DPPH radicals with the scavenging rates reached to 80.26% and 83.57%,respectively.

关 键 词：银杏 抗氧化肽 分离纯化 飞行时间质谱 

分 类 号：S664.3[农业科学—果树学] TS201.2[农业科学—园艺学]