野生牛肝菌保健功能研究  

Study on Health Function of Natural Boletus

作　　者：刘佳[1] 高敏[1] 吴克枫[1] 阮海星[1] 王雨[1] 俞红[1] 

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004

出　　处：《中国卫生工程学》2011年第3期224-226,共3页Chinese Journal of Public Health Engineering

摘　　要：目的观察野生牛肝菌对昆明种小鼠免疫功能的影响和对SD大鼠的降血脂作用。方法 (1)免疫功能实验:实验设野生牛肝菌组9.0 g/(kg bw)+环磷酰胺(CP)、CP组、正常对照组。灌胃小鼠牛肝菌提取液35 d,CP腹腔注射造成小鼠免疫损伤。观察小鼠胸腺脏器系数、血清溶血素、小鼠足趾DTH、碳粒廓清指数α等指标。(2)降血脂实验:设3个野生牛肝菌剂量组4.0、8.0、16.0 g/(kg bw)、阴性对照组和高脂模型对照组。各剂量组大鼠灌胃牛肝菌提取液30 d,对照组灌胃等量蒸馏水,在试验结束时取大鼠血清测定TG、CHO、LDH 3个指标。结果 (1)免疫功能试验中免疫损伤的小鼠血清溶血素(HC50)、小鼠足趾DTH、碳粒廓清指数α明显高于CP组,差异有统计学意义(P<0.01),其中小鼠足趾DTH恢复至正常对照组水平。(2)各剂量组的血脂指标与高脂对照组差异无统计学意义(P>0.01)。结论在本实验条件下,野生牛肝菌对CP所致的免疫损伤小鼠细胞免疫、体液免疫、非特异性免疫功能有明显改善作用,但不能预防大鼠的高脂血症。Objective To study the immune regulation of natural Boletus on the cyclophosphamide（CP）-induced mouse immune injury and the effect of the blood lipids reduction function of SD rats of natural Boletus.Methods（1） The immune regulation tests： Mice were randomly divided into three groups： the normal control,9g/kg.bw Boletus ＋ 50 mg/kg.bw CP,and 50 mg/kg.bw CP.They were administered by oral gavage for 35 days,then CP was injected into their abdominal cavity,induced their immune injury.The relative immune indexes such as clinic symptom,organic index,hemolysin（HC50）,footpad delayed type hypersensitivity（DTH）,and α-index of carbon clear test were observed.（2） The blood lipids reduction function tests： Rats were randomly divided into 5 groups： 3 test groups administered with natural Boletus（4.0 g/kg.bw,8.0 g/kg.bw,16.0 g/kg.bw respectively）,negative control group,and positive control group.The indices were detected including TG,CHO and LDH.Results（1） Thymus index of mice,HC50 in serum,delayed type hypersensitivity（DTH）,and the carbon clearance index（α） of carbon clearance test in natural Boletus（9.0 g/kg.bw）＋CP groups were significantly more than that in positive control group（CP）（P0.01）.The delayed type hypersensitivity（DTH）have restored the normal test group level.（2） There were no significant difference in TG,CHO and LDH between the test groups and positive control group.Conclusion In our laboratory,the results demonstrate that natural Boletus has significant melioration in mouse humoral,cellular and innate immune injury induced by CP.But this study also shows that natural Boletus does not protect the rats from the high blood lipids.

关 键 词：野生牛肝菌 小鼠 SD大鼠 保健功能 

分 类 号：R282.7[医药卫生—中药学]