重组鼠李糖乳杆菌发酵L-乳酸的初步研究  

Preliminary Study on the Production of L-lactic acid by Recombinant Lacbacillus rhamnosus

作　　者：郭铃[1] 孙靓[1] 李检秀[1] 吴军华[2] 黄艳燕[1] 孙菲菲[1] 黄日波[1,2] 

机构地区：[1]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物炼制重点实验室,广西南宁530007 [2]广西科学院,广西南宁530007

出　　处：《酿酒科技》2011年第6期21-24,共4页Liquor-Making Science & Technology

基　　金：国家科技支撑计划项目(2007BAD75B06);广西科学基金(桂科攻0782003-4)

摘　　要：将自杀质粒pDT转化到鼠李糖乳杆菌JCM1553中,成功获得了具有四环素抗性的鼠李糖乳杆菌。生长曲线说明,重组菌株能在5μg/mL四环素MRS培养基中正常生长,最适培养温度为37～42℃。重组菌株在含有10%、15%、20%、25%的葡萄糖发酵培养基中的发酵结果显示:37℃,200 r/min发酵40～88 h,菌体密度OD600最大为29.05;残糖含量最低为0.604%,L-乳酸的最大产量为228.19 g/L,葡萄糖转化率最大为96.24%。The suicide plasmid pDT was transformed into Lactobacillus rhamnosus JCM 1553,then a recombinant stain with tetracycline resistance had been constructed successfully.The growth curve of the recombinant Lacbacillus rhamnosus showed that the strain could reproduce regularly in MRS with 5 μg/mL tetracycline,and its optimum culture temperature was at 37～42 ℃.After 40～88 h fermentation in Erlenmeyer flasks containing 10 %,15 %,20 %,25 % glucose at 37 ℃/200 rpm by recombinant stain,the highest yield of L-lactic acid was 228.19 g/L,the highest conversion rate of glucose was 96.24 %,OD600 of the strain achieved 29.05,and the lowest residual sugar content was 0.604 %.

关 键 词：微生物 同源重组 鼠李糖乳杆菌 发酵L-乳酸 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]