受体三阴性乳腺癌中CK14和EGFR的检测及临床意义  

Detection of CK14 and EGFR in Triple-negative Breast Carcinoma and Its Clinical Significance

作　　者：赵鸿[1] 薛娣[2] 冷冬妮[3] 

机构地区：[1]南京医科大学第一附属医院检验科,江苏南京210029 [2]江苏省镇江市第一人民医院病理科,江苏镇江212002 [3]解放军第454医院病理科,江苏南京210002

出　　处：《临床医学工程》2011年第6期862-863,共2页Clinical Medicine & Engineering

摘　　要：目的研究三阴性乳腺癌中CK14和EGFR蛋白的表达及意义。方法免疫组化法检测95例三阴性及50例非三阴性浸润性乳腺癌中CK14和EGFR蛋白的表达,运用荧光原位杂交(FISH)检测4例EGFR基因扩增情况。结果所有三阴性乳腺癌病例显示为高级别癌,核分裂像易见。肿瘤组织具有推进式的边缘,周围伴淋巴细胞浸润。瘤细胞呈合体细胞样,通常见坏死。免疫组化示ER、PR和HER2均阴性,CK14和EGFR在三阴性乳腺癌中的表达率分别为83.2%和65.3%,在非三阴性乳腺癌中的表达率分别为2.0%和20.0%(P<0.01)。4例FISH检测见EGFR基因扩增。结论三阴性乳腺癌具有独特的组织学与基底样细胞表型,EGFR常过度表达,对于EGFR分子靶向治疗可能有效。Objective To investigate the expression of CK14,EGFR in triple-negative invasive breast carcinoma and its significance. Methods 95 cases of triple-negative tumours and 50 cases of non-triple-negative tumours were tested by immunohistochemical method. EGFR gene in 4 cases were tested by fluorescence in situ hybridization（FISH） . Results All triple-negative invasive breast carcinoma were high grade with a very high mitotic index,the tumors had pushing margins with a peri-tumoural lymphocytic infiltrate and had a syncytial growth pattern. All triple-negative cases were negative for ER,PR and HER2,83.2% were positive for CK14 and 65.3% were positive for EGFR,while only 2.0% CK14 and 20.0% EGFR were positive in non-triple-negative tumours（P 0.01） . FISH test of EGFR in 4 cases showed gene amplification. Conclusion Triple-negative breast carcinoma has unique histological and basal features. The EGFR protein is often overexpressed in this subtype,so the patients may benefit from the treatment with anti-EGFR drugs.

关 键 词：三阴性乳腺癌 免疫组化 FISH CK14 EGFR 

分 类 号：R737.9[医药卫生—肿瘤]