高能脉冲电子束诱变筛选高产酒精酵母及其发酵条件优化  

Mutation and screening of high-alcoholic-yield yeast by HEPE and optimization of the fermentation condition

作　　者：韩晶晶[1] 吕江涛[1] 张琴[1] 王言[1] 付玉洁[1] 付海英[2] 汪世龙[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《辐射研究与辐射工艺学报》2011年第3期154-158,共5页Journal of Radiation Research and Radiation Processing

摘　　要：为获得更高酒精产量的菌株,以高能脉冲电了束为诱变源,以酿酒酵母Saccharomyces Cerevisiae YE0为出发菌株,通过乙醇胁迫筛选和气相色谱法测定酒精浓度,筛选得到一株性状优良的高产酒精酵母YF1。运用正交试验对诱变菌株发酵条件进行优化并比较其与出发菌株YE0的生长曲线和热致死温度,结果表明:在发酵温度34℃,发酵时间72h,发酵培养基的葡萄糖浓度30%时,酒精产量达到15.57%,较出发菌株YE0(9.84%)提高58.23%;诱变菌株YF1生长速度和热致死温度相对出发菌株YE0均有明显的提高。诱变菌株YF1也可作为进一步诱变筛选的出发菌株,获得更高酒精产量的菌株。The Saccharomyces Cerevisiae YE0 was mutated using high-energy-pulse-electron （HEPE） beam. After ethanol stress and determination of the alcohol yield by gas chromatographs, the mutant YF1 with high alcoholic yield was obtained. The results showed that under the optimized fermentation conditions （34℃ as the fermentation temperature, 72 h as the fermentation time and 30% as the glucose concentration）, the alcoholic yield of YF 1 was 15.57% which was 58.23% higher than that of the original strain YE0（9.84%） under the same conditions. The growth rate and lethal temperature of the mutant YF1 were obviously enhanced to the original strain YE0. The mutant YF1 has a great potential application in industrial production of alcohol. And it can also be used as the original strain for further mutagenesis to get the strain of higher alcoholic yield.

关 键 词：高能脉冲电子束 酿酒酵母 高产酒精 

分 类 号：Q345.1[生物学—遗传学]