p53 antibodies,metallothioneins,and oxidative stress markers in chronic ulcerative colitis with dysplasia  

作　　者：Hala E Hamouda Soha S Zakaria Saber A Ismail Mahmoud A Khedr Wael W Mayah 

机构地区：[1]Department of Medical Biochemistry,Faculty of Medicine,Tanta University [2]Department of Tropical Medicine,Faculty of Medicine,Tanta University

出　　处：《World Journal of Gastroenterology》2011年第19期2417-2423,共7页世界胃肠病学杂志（英文版）

摘　　要：AIM:To investigate the role of p53 antibodies (p53Abs),metallothioneins (MTs) and oxidative stress markers in the early detection of dysplasia in chronic ulcerative colitis (UC).METHODS:The study included 30 UC patients,15 without dysplasia (group Ⅱ) and 15 with dysplasia (group Ⅲ),in addition to 15 healthy volunteers (group Ⅰ,control subjects).The enzyme-linked immunosorbent assay technique was used to measure serum p53Abs and MTs,while advanced oxidation protein products (AOPPs),and reduced glutathione (GSH) levels were measured by spectrophotometric method in all subjects.RESULTS:In group Ⅱ and group Ⅲ compared to group Ⅰ,there were significant increases in serum levels of AOPPs (145.94 ± 29.86 μmol/L and 192.21 ± 46.71 μmol/L vs 128.95 ± 3.06 μmol/L,P < 0.002 and P <0.001,respectively),MTs (8.18 ± 0.35 μg/mL and 9.20 ± 0.58 μg/mL vs 6.12 ± 0.25 μg/mL,P < 0.05 and P < 0.05,respectively),and p53Abs (20.19 ± 3.20 U/mL and 34.66 ± 1.34 U/mL vs 9.42 ± 1.64 U/mL,P < 0.001 and P < 0.001,respectively).There were significantly higher levels of AOPPs (P < 0.05) and p53Abs (P < 0.001) in UC patients with dysplasia compared to those without dysplasia,while MTs showed no significant difference between the 2 groups (P > 0.096).In contrast,GSH levels showed a significant decrease in both patients' groups (1.87 ± 0.02 μmol/mL and 1.37 ± 0.09 μmol/mL vs 2.49 ± 0.10 μmol/mL,P < 0.05 and P < 0.05 in groups Ⅱ and Ⅲ,respectively) compared with group Ⅰ,and the levels were significantly lower in group Ⅲ than group Ⅱ (P < 0.05).There was a positive correlation between AOPPs and both MTs (r=0.678,P < 0.001) and p53Abs (r=0.547,P < 0.001),and also between p53Abs and MTs (r=0.739,P < 0.001).There was a negative correlation between AOPPs and GSH (r =-0.385,P < 0.001),and also between GSH and both MTs (r=-0.662,P < 0.001) and p53Abs (r=-0.923,P < 0.001).CONCLUSION:Oxidative stress and oxidative cellular damage play an important role in the pathogenesis of chronic UC and the associated carcinogenAIM：To investigate the role of p53 antibodies （p53Abs）,metallothioneins （MTs） and oxidative stress markers in the early detection of dysplasia in chronic ulcerative colitis （UC）.METHODS：The study included 30 UC patients,15 without dysplasia （group Ⅱ） and 15 with dysplasia （group Ⅲ）,in addition to 15 healthy volunteers （group Ⅰ,control subjects）.The enzyme-linked immunosorbent assay technique was used to measure serum p53Abs and MTs,while advanced oxidation protein products （AOPPs）,and reduced glutathione （GSH） levels were measured by spectrophotometric method in all subjects.RESULTS：In group Ⅱ and group Ⅲ compared to group Ⅰ,there were significant increases in serum levels of AOPPs （145.94 ± 29.86 μmol/L and 192.21 ± 46.71 μmol/L vs 128.95 ± 3.06 μmol/L,P 0.002 and P 0.001,respectively）,MTs （8.18 ± 0.35 μg/mL and 9.20 ± 0.58 μg/mL vs 6.12 ± 0.25 μg/mL,P 0.05 and P 0.05,respectively）,and p53Abs （20.19 ± 3.20 U/mL and 34.66 ± 1.34 U/mL vs 9.42 ± 1.64 U/mL,P 0.001 and P 0.001,respectively）.There were significantly higher levels of AOPPs （P 0.05） and p53Abs （P 0.001） in UC patients with dysplasia compared to those without dysplasia,while MTs showed no significant difference between the 2 groups （P 0.096）.In contrast,GSH levels showed a significant decrease in both patients＇ groups （1.87 ± 0.02 μmol/mL and 1.37 ± 0.09 μmol/mL vs 2.49 ± 0.10 μmol/mL,P 0.05 and P 0.05 in groups Ⅱ and Ⅲ,respectively） compared with group Ⅰ,and the levels were significantly lower in group Ⅲ than group Ⅱ （P 0.05）.There was a positive correlation between AOPPs and both MTs （r=0.678,P 0.001） and p53Abs （r=0.547,P 0.001）,and also between p53Abs and MTs （r=0.739,P 0.001）.There was a negative correlation between AOPPs and GSH （r =-0.385,P 0.001）,and also between GSH and both MTs （r=-0.662,P 0.001） and p53Abs （r=-0.923,P 0.001）.CONCLUSION：Oxidative stress and oxidative cellular damage play an important role

关 键 词：Ulcerative colitis Advanced oxidation protein products Reduced glutathione METALLOTHIONEIN 

分 类 号：R574.62[医药卫生—消化系统]