茶多酚对Hep-G2细胞活性及形态的影响  

Effects of Tea Polyphenols on Activity and Morphology of Hep-G2 Cell

作　　者：孙警辉[1] 伍春莲[1] 陈杨琼[1] 耿文峰[1] 张思楠[1] 

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2011年第2期146-150,共5页Journal of China West Normal University(Natural Sciences)

基　　金：西华师范大学科研启动项目(06B026);四川省教育厅青年基金项目(07ZB089)

摘　　要：大量研究表明,长期饮茶尤其绿茶者,可大大降低各种癌变的发病率,而且对多种体内外肿瘤具有直接杀伤作用.作者通过采用不同浓度的茶多酚作用于体外培养的肝癌细胞株Hep-G2细胞,初步探讨茶多酚对Hep-G2细胞的凋亡作用.采用MTT法(噻唑蓝比色法)和AO-PI(吖啶橙-碘化丙啶)双重荧光染色法研究了茶多酚诱导Hep-G2细胞株的凋亡作用.MTT法研究结果表明,在一定的浓度范围内,茶多酚对Hep-G2细胞有明显的抑制作用,且存在良好的剂量效应关系,随着茶多酚浓度的升高,其对Hep-G2细胞的抑制率也升高.Hep-G2细胞经茶多酚作用后,细胞形态发生明显的变化,可见凋亡小体,荧光强度增强,细胞呈现凋亡现象.茶多酚在体外对Hep-G2细胞的增殖具有抑制作用,诱导其发生凋亡.研究结果为茶多酚的进一步开发应用奠定理论基础.Many studies indicated that long-term tea drinking,especially the drinking of green tea,could not only reduce the incidence of various cancers greatly,but also have direct killing effects on a variety of tumor in vivo and in vitro.To verify the effects of tea polyphenols（TP） on apoptosis in vitro,the effects of TP on apoptosis of Hep-G2 cell were evaluated by MTT（MTT assay） and AO-PI（acridine orange-propidium iodide） double fluorescence staining.The results of MTT assay showed that in a certain concentration range,TP inhibited significantly the growth of Hep-G2 cell,and the inhibitory effect showed good dose-response relationship with the TP concentration.With raising the TP concentration,its inhibitory rate of Hep-G2 cell increased.After treated by TP,morphological changes of Hep-G2 could be observed obviously,with apoptotic bodies appeared and fluorescence intensity enhanced,which indicated that apoptosis occurred in these cells.The results suggested that TP could inhibit the proliferation of Hep-G2 and induce its apoptosis in vitro.This work will give rise to the application of TP in cancer prevention.

关 键 词：茶多酚 MTT AO/PI Hep-G2细胞 细胞凋亡 

分 类 号：R151[医药卫生—营养与食品卫生学]