黑曲霉HQ-1产羧甲基纤维素酶的发酵条件优化  

Optimization of Solid Fermentation Conditions of Aspergillus niger HQ-1 to Produce Carboxymethyl Cellulase

作　　者：张辉[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《酿酒科技》2011年第5期27-31,共5页Liquor-Making Science & Technology

基　　金：山东省自然科学基金(ZR2010CQ042)

摘　　要：采用单因素试验和响应面法对黑曲霉(Aspergillus niger)HQ-1产羧甲基纤维素酶(CMC酶)的固体发酵条件进行了优化。结果表明,产酶的最佳发酵条件为:玉米秸秆粉6.0 g、麦麸6.0 g(、NH4)2SO4 1.5 g、KH2PO4 1.6 g、Mg-SO4.7H2O 0.8 g、含水量73.9%、起始pH3.93、培养温度和培养时间分别为34.1℃和96 h。优化后,菌株产CMC酶活力最高为317.103 U/g,比未优化的酶活力最高值(65.507 U/g)提高了3.84倍。The solid fermentation conditions of Aspergillus niger HQ-1 to produce carboxymethyl cellulase were optimized by single-factor test and response surface method （RSM） as follows： corn stover powder 6.0 g, wheat bran 6.0 g, （NH4）2SO4 1.5 g, KH2PO4 1.6 g, MgSO4.7H2O 0.8 g, moisture content 73.9 %, initial pH 3.93, culture temperature and time were at 34.1 ℃ and 96 h respectively. After the optimization, the activity of produced carboxymethyl cellulase could reach to 317.103 U/g, 3.84 times higher than carboxymethyl cellulase activity （65.507 U/g） under the unoptimized conditions.

关 键 词：微生物 黑曲霉 羧甲基纤维素酶 响应面法 优化 固体发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]