小鼠胚胎神经管发育过程中Rho A和Rac 1的表达及其意义  

The expression and significance of Rho A and Rac1 in the development of mouse neural tube

作　　者：张慧[1] 李秀国[2] 崔运河[1] 孔佑华[1] 王凤琴[1] 郭岩[1] 

机构地区：[1]济宁医学院基础医学与法医学院,山东济宁272067 [2]济宁医学院附属第一人民医院,济宁272011

出　　处：《济宁医学院学报》2011年第3期153-155,共3页Journal of Jining Medical University

基　　金：济宁医学院2008年青年科学基金资助项目

摘　　要：目的探讨Rho A、Rac 1在小鼠胚胎神经管发育过程中的作用及意义。方法应用免疫组织化学染色和图像分析方法,检测Rho A、Rac 1蛋白的表达,分析在不同胎龄小鼠胚胎神经管上皮及其周围间充质的分布状况和变化规律。结果在小鼠胚胎神经管发育过程中,Rho A在胚胎神经管上皮及其周围间充质都有表达;Rac 1仅在孕7.5 d和8.5 d小鼠胚胎神经管腔面表达,而在孕9.5 d和10.5 d,Rac 1表达是阴性的。RhoA和Rac 1的表达都呈现规律性变化:Rho A在整个神经管发育过程中呈现逐渐下降的趋势;Rac 1仅在神经管关闭期间表达,神经管关闭后,Rac 1的表达呈阴性。结论 Rho A和Rac 1直接或间接参与了小鼠胚胎神经管形态构建过程,并且在神经管关闭期间起着重要的调节作用。Objective To explore the role and significance of Rho A and Rac 1 in the development of neural tube of the mouse embryos.Methods Rho A and Rac 1 were measured using immunohistochemistry and image analysis to analyse the distribution and changes in the epithelium of embryonic neural tube and surrounding mesenchyme of mouse with different fetal age.Results Rho A was expressed in the epithelium of embryonic neural tube and surrounding mesenchyme during the development of the embryonic neural tube of mice.Rac 1 was expressed in the neurocoel from 7.5-day to 8.5-day gestition mouse while it was negative in the mouse embryonic neural tube from 9.5-day to 10.5-day gestition mouse.Variations of the expression of Rho A and Rac 1 were regular in the development of embryonic neural tube in the mouse： the expression level of Rho A was gradually decreasing during the development of the neural tube,and Rac 1 was expressed in the formation of the neural tube.After the formation of the neural tube the expression of Rac 1 was negative.Conclusion Rho A and Rac 1 were involved in morphogenesis of embryonic neural tube directly and indirectly,and they would be the important regulator during the neural tube formation.

关 键 词：RHO A RAC 1 神经管 发育 形态构建 小鼠 

分 类 号：Q954.48[生物学—动物学] R-332[医药卫生]