紫外线和^(60)Co辐照诱变选育高效嗜酸氧化亚铁硫杆菌  

Mutagenesis of Acidithiobacillus ferrooxidans by ultraviolet radiation and ^(60)Co-y ray for high Fe^(2+) oxidation rate

作　　者：刘艳华[1] 张朝正[2] 吴效楠[3] 寇丽[1] 郑宇[2] 

机构地区：[1]天津科技大学理学院,天津300457 [2]工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 [3]承德石油高等专科学校化工系,承德067000

出　　处：《工业微生物》2011年第3期81-85,共5页Industrial Microbiology

摘　　要：依次利用紫外线和^(60)Co-γ射线辐照诱变的方法对嗜酸氧化亚铁硫杆菌进行诱变,选育高效嗜酸氧化亚铁硫杆菌菌株。结果表明,紫外诱变可以有效提高嗜酸氧化亚铁硫杆菌的Fe^(2+)氧化速率,最佳紫外线诱变时间为240 s。诱变后茵株的Fe^(2+)氧化速率从0.273 g/L/h提高到了0.312g/L/h。继续利用^(60)Co-γ射线进行辐照诱变,Fe^(2+)氧化速率提高到了0.342g/L/h,最易出现正突变的辐照吸收剂量为0.5 kGy～1.5 kGy。经过两次诱变后,菌株的Fe^(2+)氧化速率比出发菌株提高了25.7%。诱变后的菌株在培养基pH为1.8时产生较少的沉淀(2.26 g/L),并保持较高的Fe^(2+)氧化速率(0.337 g/L/h),具有良好的工业化应用前景。The mutation of Acidithiobacillus ferrooxidans by ultraviolet radiation and 60 Co-γ ray for selecting mutants with high Fe2＋ oxidation rate was carried out. The results showed that the Fe2＋ oxidation rate of A. ferrooxidans was improved from 0. 273 g/L/h to 0. 312 g/L/h after ultraviolet radiation of 240 s, which was the optimal duration for ultraviolet mutation. Subsequently, the Fe2＋ oxidation rate was enhanced to 0. 342 g/L/h by 60 Co-γ ray. When the radiation absorbed dose ranged from 0.5 kGy to 1.5 kGy, there were more positive mutants. As a result, the Fe2＋ oxidation rate of A. ferrooxidans was improved by 25. 7% when compared with that of the original strain. Furthermore, when the media pH was 1.8, the mutant strain produced less precipitate （2.26 g/L） and the Fe2＋ oxidation rate （0. 337 g/L/h） was also acceptable. These results showed the mutant strain had a potential application in industry.

关 键 词：嗜酸氧化亚铁硫杆菌 诱变 紫外线 60Co-γ射线 氧化速率 

分 类 号：Q933[生物学—微生物学]