ADAR1同工型基因在小鼠急性T淋巴细胞白血病模型中的表达  

Expression of ADAR1 Isoforms in Murine Acute T-ALL Leukemia Model

作　　者：马翠花 田晨 种靖慧 师迎旭 王金宏 林永敏 许静 郑国光 

机构地区：[1]中国医学科学院、北京协和医学院,血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2011年第3期566-569,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号30971111);天津市自然科学基金资助项目(编号11JCZDJC18200);国家973项目(编号2009CB521803,2009CB918901);教育部新世纪优秀人才计划(编号NCET-08-0329)

摘　　要：本研究探讨RNA编辑酶ADAR1的2种同工型P110和P150在小鼠白血病发展中的表达变化规律。采用Notch1过表达小鼠急性T淋巴细胞白血病移植模型,在发病不同阶段分离骨髓单个核细胞,并在发病晚期用流式细胞术分选CD45.2+GFP+白血病细胞,用实时定量PCR方法检测ADAR1的表达变化。结果表明:对照组和白血病组小鼠骨髓细胞均表达ADAR1的2种同工型P110和P150mRNA;Notch1过表达导致的小鼠白血病发展过程中2种同工型的表达水平变化不同;随着白血病的发展,P110的表达水平逐渐升高,而P150表达水平逐渐降低,在移植后的第14天降至对照组的1/4。分选后的CD45.2+GFP+白血病细胞高表达P110而低表达P150。结论 :ADAR1的亚型P110和P150mRNA在小鼠白血病中的表达变化规律存在差异,提示二者介导的RNA编辑可能在白血病发展中发挥不同作用。This study was purposed to investigate the expression of ADAR1 isoforms of PIIO and PI50 during the development of murine leukemia. A Notchl over-expressing murine T cell acute lymphoblastic leukemia model was used to study the expression of ADAR1. BMMNC were isolated at different stages of disease and CD45.2 ＋ GFP ＋ leukemia cells were sorted by flow cytometry at late stage. The expression of ADAR1 was detected by real time quantitative PCR. The results showed that mouse bone marrow cells from both leukemia and control groups expressed PllO and P150. Difference of Pl10 and P150 mRNA expression were observed during the development of leukemia. The expression of P110 dramatically increased and was significantly higher than that in control group. However, the expression level of P150 in leukemia group decreased stably and reached one-fourth of that in control group at 14 day. Furthermore, similar expression patterns could be detected in sorted CD45. 2＋ GFP＋ leukemia cells. It is concluded that the mRNA expressions of PllO and P150 show diverse patterns in the development of leukemia, suggesting that RNA editing mediated by ADAR1 isoforms may play different roles in leukemia.

关 键 词：RNA编辑 ADAR1 P110P150 急性T淋巴细胞白血病:白血病模型 

分 类 号：R733.7[医药卫生—肿瘤]