玉米须中抑制黄嘌呤氧化酶活性组分的筛选及其作用  

Screening of active ingredient of Stigmata maydis inhibitrg xanthine oxidase and its effect

作　　者：姜彤伟[1] 徐建 王晓明[3] 陈雪松[4] 邱智东[1] 

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]吉林省修正药业集团,吉林长春130012 [3]吉林大学第一医院心内科,吉林长春130021 [4]吉林省创智医药开发有限公司

出　　处：《吉林大学学报（医学版）》2011年第3期433-436,共4页Journal of Jilin University：Medicine Edition

基　　金：国家科技部重大新药创制基金资助课题(2009ZX09103-348)

摘　　要：目的:提取玉米须中具有抑制黄嘌呤氧化酶(XOD)作用的活性组分,为进一步开发治疗痛风的中药新药提供依据。方法:通过测定黄嘌呤-黄嘌呤氧化酶体系产生尿酸和超氧离子的生成量,得出玉米须不同溶剂提取物(乙醚提取物、乙酸乙酯提取物、正丁醇提取物和水提取物)及不同溶剂(水和10%、30%、50%、70%、90%乙醇)纯化得到的活性组分对XOD活性抑制率,以确定活性组分,并将其与阳性对照药别嘌醇进行XOD活性半数抑制率(IC50)比较。结果:乙酸乙酯提取物XOD活性抑制率最高,为(25.60±1.01)%;与其他各组比较,经50%乙醇纯化得到的活性组分XOD活性抑制率最高,为(60.90±1.27)%。与阳性药别嘌醇进行比较,通过测定尿酸生成量得出50%乙醇纯化得到的活性组分对XOD的IC50为6.00 mg.L-1,别嘌呤醇对XOD的IC50为1.10 mg.L-1;而通过测定超氧离子生成量得出50%乙醇纯化得到的活性组分对XOD的IC50为4.50 mg.L-1,别嘌呤醇对XOD的IC50为1.05 mg.L-1。结论:玉米须中抑制XOD的活性组分为经50%乙醇纯化得到的活性组分。Objective To extract the active ingredient of Stigmata maydis for inhibiting the xanthine oxidase（XOD）and provide the basis for the further development of new Chinese medicine in treatment of gout.Methods The production of uric acid and superoxide generation were detemined,and the inhibitory rates of XOD after treated with different solvent extraction and different solvent depuration of Stigma maydis were calculated,then the data was compared with positive control allopurinol.Results Compared with other groups,the inhibitory rate of XOD activity of active component purified by 50% ethanol was the highest（60.90%±1.27%）.The IC50 of the production of uric acid was 6.0 mg·L-1 and that of positive control allopurinol was 1.10 mg·L-1.The IC50 of the production of superoxide ions was 4.5 mg·L-1,and that of positive control allopurinol was 1.05 mg·L-1.Conclusion The active ingredient of Stigma maydis which could inhibit XOD activity is purified by 50% ethanol.

关 键 词：玉米须 痛风 黄嘌呤氧化酶 

分 类 号：R285.5[医药卫生—中药学] R589.7[医药卫生—中医学]