LAMP技术在检测原料乳及乳制品致病菌方面的应用  

Application of LAMP technique for detection of pathogenic bacteria in raw milk and dairy products

作　　者：胡惠秩[1,2] 满朝新 蒋亚男[2] 杨士芹[2] 谢鲲昊[2] 姜毓君[1,2] 

机构地区：[1]国家乳业工程技术研究中心,哈尔滨150086 [2]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2011年第6期55-57,共3页China Dairy Industry

基　　金：国家科技支撑计划项目(2009BADB9B06)

摘　　要：环介导等温扩增(LAMP,Loop-Mediated Isothermal Amplification)作为一种新的检测技术,具有操作简便、快速、特异性强等优点,目前已在原料乳及乳制品致病菌的检测中得到初步应用,本文综述了这一技术的原理、鉴别及检测的方法,及其在原料乳及乳制品检测方面的应用进展。LAMP（Loop-Mediated Isothermal Amplification） as a novel method has been used to rapidly detect pathogenic bacteria in raw milk and dairy products.Its advantages include easy operation,rapid detection and strong specificity,which is the reason to be widely used to detect pathogenic bacteria in dairy at present.This paper reviewed the principle,identifying and detection methods of LAMP,as well as its applications in raw milk and dairy products detection.

关 键 词：LAMP 原料乳及乳制品 检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]