SO_2诱导驯化城市污水处理厂氧化沟微生物的多样性分析  

Diversity Analysis of Desulfuration Bacterium from the Oxidation Ditch of City Sewage Treatment Plant with SO_2 Gas

作　　者：黄兵[1] 张世玲[2] 章江洪[3] 敖勇 施哲[5] 

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650093 [2]昆明冶金高等专科学校材料与机械学院,昆明650033 [3]昆明理工大学理学院,昆明650093 [4]云南省建筑材料科学研究设计院,昆明650022 [5]昆明理工大学冶金与能源工程学院,昆明650093

出　　处：《环境科学》2011年第7期2132-2137,共6页Environmental Science

基　　金：国家自然科学基金项目(50068002)

摘　　要：对城市污水处理厂氧化沟采集的微生物用低浓度SO2诱导驯化6 d以上可培养出脱SO2微生物种群,它们之间相互协同,共同行使着对溶于水中的SO2生化降解的生物学功能,对SO2的生化降解速率高达888 mg.(L.h)-1,1.5 h水中的SO32-的降解效率>85%.脱硫菌样品经DNA提取及16S rRNA基因片段的聚合酶链反应(PCR)扩增,构建出脱硫菌16S rRNA基因片段克隆文库和2棵系统发育树.其基因片段序列的系统发育分析表明,起主要脱硫作用的8种优势菌,占整个菌群的69%,其中有4种为α-变形菌纲,4种为β-变形菌纲,部分菌与已发现的一些脱硫菌有较高的相似度,但不是同一种.这些优势脱硫菌的16S rRNA基因序列信息结果和驯化方法为含硫烟气的微生物脱硫提供了寻找脱硫菌源基础和驯化脱SO2菌的方法.A group of removing SO2 bacterium was obtained from the oxidation ditch of city sewage treatment plant by inductive domestication over 6 d with low concentration SO2 gas, and they have an ability with biodegradation rate of 888 mg· （L·h）^ - 1 and a degradation efficiency of 85% during 1.5 h for SO2 dissolved in water with their synergy. The clone library and two phylogenetic trees of the removing SOs bacterium communities were obtained based on 16S rRNA DNA comparison by DNA extraction of the sample and in situ polymerase chain reaction （PCR）. The phylogenetic analysis showed that 8 dominant desulfuration bacterium occupy about 69% of all removing SO2 bacterium, and some of them have a kindred with discovered desulfuration bacterium but not homogeneity, and there are four belong to ct-Proteobacteria, another four belong to fl-Proteobacteria in them. The gene information about 16S rRNA sequence of the dominant desulfuration bacteria and domestication method provide a basic of looking for or domesticating removing SO2 bacterium for development microbial desulfurization technology of contained SOs tail gas.

关 键 词：脱硫菌 16S rRNA基因测序 二氧化硫 驯化 微生物多样性 

分 类 号：X172[环境科学与工程—环境科学]