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机构地区:[1]武汉工程大学真菌生理学实验室,武汉430074
出 处:《湖北农业科学》2011年第11期2247-2249,共3页Hubei Agricultural Sciences
摘 要:采用水浸提法和乙醇萃取法制备大蒜素,对稻瘟病菌(Pyricularia oryzae)、小麦纹枯病菌(Rhizocto-nia cerealis)、油菜菌核病菌(Sclerotinia sclerotiorum)进行体外抑制试验,结果表明,水浸提法制备的20mg/mL大蒜素对3种真菌的抑菌效果分别达到70.6%、83.6%、94.3%,乙醇萃取法制备的20 mg/mL大蒜素的抑菌效果分别达到79.7%、89.7%、93.0%,供试最低抑菌浓度5 mg/mL大蒜素的抑菌效果分别达到29.0%、61.0%、93.7%,显示大蒜提取液对稻瘟病菌、小麦纹枯病菌、油菜菌核病菌都有较强的抑制作用,对油菜菌核病菌的抑制作用最强。结果提示,大蒜提取液对植物病原真菌抑制作用的强弱因供试菌、提取方法、作用浓度而异,这为开发农用抗生素对农作物病害进行生物防治提供了实践依据。In vitro activity of garlic against Pyricularia oryzae,Rhizoctonia cerealis,Sclerotinia sclerotiorum was tested.The results showed tha for the garlic prepared from water leach,the inhibitory effect on P.oryzae,R.cerealis,S.sclerotiorum was 70.6%,83.6% and 94.3%,respectively;while that for the garlic prepared from achohol extraction was 79.7%,89.7% and 93.0% respectively.The antifungal activity of garlic at MIC,5mg/mL,on the three fungi was 29.0%,61.0% and 93.7% respectively,which meant that garlic extraction has strong inhibitory effect against the three fungi.The inhibitory effect of garlic against plant pathogenic fungi differed according to different fungi,extrating method and concentration;and this offered a base for developing agro-antibiotic for bio-controling of crop diseases.
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