叶黄素对小鼠脑缺血/再灌注损伤所致炎症反应的影响  

EFFECT OF LUTEIN ON THE INFLAMMATION REACTION INDUCED BY CEREBRAL ISCHEMIA/REPERFUSION INJURY IN MICE

作　　者：孙雅煊[1,2] 刘婷[3] 戴雪伶[1] 高兆兰[1] 惠伯棣[1] 姜招峰[1] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]中国科学院寒区旱区环境与工程研究所,兰州730000 [3]石河子大学药学院,石河子832003

出　　处：《营养学报》2011年第3期282-285,共4页Acta Nutrimenta Sinica

基　　金：北京联合大学校级课题资助项目(No.zk200905x);北京市属高等学校人才强教计划资助项目(No.PHR201108422)

摘　　要：目的观察叶黄素对脑缺血/再灌注损伤及炎症反应的影响。方法将雄性小鼠随机分为5组:假手术组、模型组、叶黄素高、中、低剂量处理组。线栓法建立局灶性脑缺血/再灌注模型。2,3,5-氯化三苯基四氮唑(TTC)染色法测定脑梗死面积。采用不同方法评价脑组织病理学,变化、髓过氧化物酶(MPO)活性及细胞间粘附因子-1(ICAM-1)表达。结果与模型组比较,叶黄素剂量依赖性地减少梗死面积,改善病理学变化,降低MPO活性和ICAM-1表达。结论叶黄素可通过抑制炎症反应,减轻脑缺血/再灌注损伤。Objective To observe the effects of lutein on cerebral ischemic/reperfusion injury and inflammation reaction.Method Male ICR mice were randomly divided into five groups：model group,sham group,high,middle and low dose lutein treatment groups.The focal cerebral ischemic/reperfusion model was established with use of suture-occluded method.2,3,5-triphenyltetrazolium chloride（TTC） staining was used to measure the cerebral infarct size.The histopathological change,myeloperoxidase（MPO） activity and intercellular adhesion molecule-1（ICAM-1） expression were evaluated by various methods.Results Compared with the model group,lutein reduced the infarct size,improved the histopathological changes,and decreased the MPO activity and ICAM-1 expression in a dose-dependent manner.Conclusion Lutein has the ability to lessen cerebral ischemic/reperfusion damage by inhibiting the inflammation reaction.

关 键 词：叶黄素 脑缺血/再灌注 损伤 炎症反应 

分 类 号：R285.5[医药卫生—中药学]