花色苷对THP-1细胞中ABCG1表达的影响及机制分析  

EFFECTS OF ANTHOCYANIN ON ATP BINDING CASSETTE TRANSPORTER G1 EXPRESSION AND ANALYSIS OF THE MECHANISMS

作　　者：张英慧[1] 董华强[1] 钟希琼[1] 黄剑波[1] 

机构地区：[1]佛山科学技术学院北院生命科学学院食品系,广东南海528231

出　　处：《营养学报》2011年第3期286-290,296,共6页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.31071537)

摘　　要：目的研究花色苷矢车菊素-3-O-β-葡萄糖苷(C3G)对人巨噬细胞THP-1中ABCG1表达的影响,并分析相关作用机制。方法以C3G干预培养诱导分化的THP-1细胞,荧光实时定量RT-PCR检测ATP结合盒式转运蛋白G1(ABCG1)以及其直接调节因子肝脏X受体α(liver X receptorα,LXRα)的mRNA表达,时间分辨荧光共振能量转移(time-resolved fluorescence resonance energy transfer,TR-FRET)方法检测C3G与LXRα配体结合域的结合能力。结果 100μmol/L C3G处理THP-1细胞16 h显著增加了ABCG1 mRNA的表达(为对照组的1.98倍,P<0.05);尽管在TR-FRET试验中,C3G并未呈现典型的配体结合曲线,而50μmol/L和100μmol/L C3G显著增加了THP-1细胞中LXRαmRNA的表达(分别为对照组的2.21倍和2.83倍,P<0.05)。结论 C3G促进了ABCG1表达,该机制可能通过增加核受体LXRαmRNA表达来实现。Objective To investigate the effects of anthocyanin cyanidin-3-O-β-glucoside（C3G） on ATP binding cassette transporter G1（ABCG1） expression in the human monocyte/macrophage cell line THP-1,and to explore the mechanisms involved.Method The differentiated THP-1 cells were treated with C3G.mRNA expression levels of ABCG1 and its direct regulator liver X receptor α（LXRα） was detected by RT-PCR.The method of time-resolved fluorescence resonance energy transfer（TR-FRET） was utilized to survey the connecting ability of C3G to LXRα ligand binding domain（LXRα-LBD）.Results Treatment of 100 μmol/L C3G for 16 h significantly increased ABCG1 mRNA expression to 1.98 folds of control（P0.05） in the differentiated THP-1 cells.Although no classical ligand binding curve of C3G was observed in TR-FRET assay,LXRα mRNA was significantly increased by 50 μmol/L and 100 μmol/L C3G treatments（2.21 and 2.83 folds of control,P0.05）.Conclusion C3G may augment ABCG1 mRNA by inducing its direct upstream regulator LXRα expression,instead of carrying out as an efficient agonist of LXRα.

关 键 词：矢车菊素-3-O-β-葡萄糖苷 ABCG1 LXRΑ 

分 类 号：R151[医药卫生—营养与食品卫生学]