牛磺酸对束缚应激小鼠焦虑状态的影响  

Anxiolytic Effect of Taurine in Stressed Mice

作　　者：陈文[1] 刘娇[1] 任超[1] 王祎[1] 龚亚京[1] 郭月逸[1] 

机构地区：[1]北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《中国食品学报》2011年第3期26-30,共5页Journal of Chinese Institute Of Food Science and Technology

摘　　要：为探讨牛磺酸对束缚应激小鼠自主探究活动能力的影响,将8周龄雄性BALB/C小鼠分为4组:正常对照组(C组)、应激对照组(S组)、低剂量牛磺酸组(LT组)、高剂量牛磺酸组(HT组)。S、LT、HT组小鼠每日给予1h的束缚刺激,30min后分别灌喂水和相应剂量的牛磺酸。连续6周后采用Smart小动物行为活动记录分析系统进行旷场和高架十字迷宫实验。旷场结果显示,S组小鼠在周边、中央区域的活动性分别比C组小鼠减弱10.8%(P<0.05)和15.7%,快速移动时间百分率显著降低(P<0.05);与S组相比较,LT和HT组小鼠在周边、中央区域的活动性都明显提升(P<0.05),不动时间百分率下降(P<0.05),慢速移动时间百分率明显上升(P<0.05)。高架十字迷宫结果显示,与C组相比较,S组小鼠的入臂总次数和在开臂的滞留时间都显著下降(P<0.05),开臂移动距离百分比下降17.5%;与S组相比较,HT组小鼠在开臂的滞留时间和移动距离百分比都明显增加(P<0.05)。以上结果提示牛磺酸能够提高束缚应激小鼠的自主活动与探究能力,缓解焦虑状态。In order to study the anxiolytic effect of taurine,8-weeks-old male BALB/C mice were divided into 4 groups：control group（C）,stressed group （S）,low-dose taurine group （LT）,and high-dose taurine group （HT） based on their body weights.The mice of S,LT and HT group were suffered restraint stress 1h a day.6 weeks later,open-field and elevated plus-maze test were determined.Open-field test results：compared with C group,fast-moving time （%） and the locomotive activity of S group in periphery and central area were markedly decreased（P0.05）;Compared with S group,LT and HT group had higher slow-moving time（%） and locomotive activity in both of periphery and central area,and had less immobility time（%） （P0.05）.Elevated plus-maze test results：compared with C group,the total entries and the percentage of time and moving distance in the open arms were markedly decreased（P0.05）;Compared with S group,HT group had a significant increase of the percentage of time and moving distance in the open arms.All the above results suggest taurine improves the locomotive activity and exploring ability of stressed mice,and shows some anxiolytic effect.

关 键 词：牛磺酸 旷场 高架十字迷宫 束缚应激 小鼠 

分 类 号：R965[医药卫生—药理学]