武汉汉族新生儿中肺表面活性物质蛋白D基因多态性及其与支气管肺发育不良相关性分析  

Polymorphisms of surfactant protein D and association between polymorphisms and bronchopulmonary dysplasia in Han racial Wuhan newborns

作　　者：周玉容[1] 常立文[1] 李文斌[1] 刘伟[1] 曾凌空[1] 张佳[1] 单瑞艳[1] 潘睿[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《中国病理生理杂志》2011年第5期968-971,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30872795);湖北省自然科学基金资助项目(No.2008CDB139);新教师基金资助项目(高等学校博士学科点专项科研基金;No.200804871058)

摘　　要：目的:研究武汉汉族新生儿中肺表面活性物质蛋白D(SP-D)基因多态性及其与支气管肺发育不良(BPD)易感性的相关关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对218例武汉汉族新生儿SP-D相关位点进行基因型检测,同时进行基因测序验证结果。结果:SP-DM et11Thr三种基因型TT、TC、CC频率分别为10.5%、49.1%、40.4%,等位基因T和C频率分别为35.1%和64.9%。SP-DA la160Thr三种基因型GG、GA、AA频率分别为54.6%、39.4%、6.0%,等位基因G和A频率分别为74.3%和25.7%。与对照组比,SP-DM et11Thr和SP-DA la160Thr基因多态性与BPD发生率无明显关联(P>0.05)。结论:SP-DM et11Thr和SP-DA la160Thr基因型和等位基因频率存在种族差异性;SP-DM et11Thr和SP-DA la160Thr基因多态性与BPD发生率无明显关联。AIM： To analyze the genetic polymorphisms of surfactant protein D（SP-D） and to investigate the association between polymorphisms and bronchopulmonary dysplasia（BPD） in Han racial Wuhan newborns.METHODS： Genotyping of SP-D polymorphisms was performed by polymerase chain reaction and restriction fragment length polymorphism analysis（PCR-RFLP） in 218 Han racial Wuhan newborns.The method of gene sequencing was used to validate the results.RESULTS： The frequencies of TT,TC,CC of SP-D Met11Thr were 10.5%,49.1% and 40.4%,respectively,and the allele frequencies of T and C were 35.1% and 64.9%,respectively.The frequencies of GG,GA,AA of SP-D Ala160Thr were 54.6%,39.4% and 6.0%,respectively,and the allele frequencies of G and A were 74.3% and 25.7%.SP-D Met11Thr and SP-D Ala160Thr displayed no significant association with BPD as compared with control group.CONCLUSION： The differences in SP-D Met11Thr and SP-D Ala160Thr polymorphisms are significant among several ethnic groups.Polymorphisms of SP-D Met11Thr and SP-D Ala160Thr display no significant association with BPD as compared with control group.

关 键 词：肺表面活性物质蛋白D 等位基因 基因型 基因多态性 支气管肺发育不良 

分 类 号：R363[医药卫生—病理学] R722[医药卫生—基础医学]