LM23 is a novel member of the Speedy/Ringo family at the ：rossroads of life and death of spermatogenic cell  

作　　者：Yi-Ming Cheng Mei-Ling Liu Meng-Chun Jia 

机构地区：[1]Graduate School of the Peking Union Medical College, Beijing 100005, China [2]Department of Reproductive Endocrinology, National Research Institute for Family Planning, Beijing 100081, China [3]Air Force Institute of Aviation Medicine, Beijing 100142, China

出　　处：《Asian Journal of Andrology》2011年第3期446-452,513,共8页亚洲男性学杂志（英文版）

基　　金：The authors thank Xin-Quan Shi, De-Yu Liu, Da-Guang Sun, Hong-Fei Xia, Qian-Xin Wang, Chong Wang, Dan Li, Xiang-Bo Xu, Yong-Jie Chang and Nan Yao for technical assistance. This work was supported by the National Natural Science Foundation of China （No. 30670784）.

摘　　要：LM23 is a gene specifically expressed in the testis of Rattus norvegicus, as previously reported by our laboratory. The aim of the study is to further investigate the biological function of LM23. Several bioinformatic tools were utilized, including PROSITE and BLAST. To determine the subcellullar localization of LM23, a polyclonal antibody specific for LM23 was generated via the immunization of rabbits. The LM23gene was cloned from rat testis tissue, and LM23 protein was expressed in Escherichia co/i. The biological function of LM23 was analyzed with microarray analysis and immunohistochemistry, using a rat model of LM23 gene knockdown. The results suggested that LM23 belongs to the Speedy/Ringo family. LM23 regulated the GI/S and G2/M transitions of the cell cycle during spermatogenesis. Downregulation of the LM23gene during spermatogenesis could lead to the activation of both the Fas-FasL pathway and the mitochondrial pathway. These novel findings indicate that LM23 has a diverse array of functions that are important in both the life and death of the spermatogenic cell.LM23 is a gene specifically expressed in the testis of Rattus norvegicus, as previously reported by our laboratory. The aim of the study is to further investigate the biological function of LM23. Several bioinformatic tools were utilized, including PROSITE and BLAST. To determine the subcellullar localization of LM23, a polyclonal antibody specific for LM23 was generated via the immunization of rabbits. The LM23gene was cloned from rat testis tissue, and LM23 protein was expressed in Escherichia co/i. The biological function of LM23 was analyzed with microarray analysis and immunohistochemistry, using a rat model of LM23 gene knockdown. The results suggested that LM23 belongs to the Speedy/Ringo family. LM23 regulated the GI/S and G2/M transitions of the cell cycle during spermatogenesis. Downregulation of the LM23gene during spermatogenesis could lead to the activation of both the Fas-FasL pathway and the mitochondrial pathway. These novel findings indicate that LM23 has a diverse array of functions that are important in both the life and death of the spermatogenic cell.

关 键 词：cell cycle LM23 SPERMATOGENESIS spermatogenic cell 

分 类 号：R[医药卫生]