φC31整合酶系统介导的位点特异性整合研究进展  

Progress of φC31 integrase system in site-specific integration

作　　者：马晴雯[1,2] 

机构地区：[1]上海交通大学附属儿童医院 [2]上海交通大学医学遗传研究所,上海200040

出　　处：《遗传》2011年第6期567-575,共9页Hereditas（Beijing)

基　　金：转基因生物新品种培育科技重大专项(编号:2009ZX08010-018B)

摘　　要：来源于链霉菌(Streptomyces)噬菌体φC31的整合酶可介导链霉菌附着位点(attB)和噬菌体附着位点(attP)之间的同源重组,这种重组亦可在多种动植物细胞内进行;而且,该整合酶还可介导含attB位点的载体以位点特异性方式整合于多种真核生物基因组内的假attP位点,并使转基因持续高效表达。因此,φC31整合酶在基因修饰、基因治疗及转基因动物研制等方面得到了广泛的应用。文章就近年来φC31整合酶整合规律、提高效率方面的改进及安全性等相关领域的研究进展进行了综述。Integrase of phage φC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP.Meanwhile,this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes.Nowadays,φC31 integrase system is becoming a potential tool for genome modification,gene therapy and transgenic research.Recent progress of φC31 integrase system in integration mode in mammalian genomes,efficiency im-provement and researches concerned on transgenic safety were summarized in this review.

关 键 词：ΦC31整合酶 位点特异性整合 基因治疗 转基因 安全性 

分 类 号：Q55[生物学—生物化学]