红托竹荪菌盖多糖的提取及抗氧化能力的研究  

Extraction and Antioxidant Activities of Polysaccharides from Pileus of Dictyophora rubrovalvata

作　　者：庄永亮[1] 孙丽平[1] 尚小丽[1] 

机构地区：[1]昆明理工大学化学与工程学院食品工程研究中心,云南昆明650224

出　　处：《林产化学与工业》2011年第3期45-49,共5页Chemistry and Industry of Forest Products

基　　金：云南省自然科学基金(2010ZC032)

摘　　要：研究了不同因素对竹荪菌盖多糖提取效果的影响,并对菌盖多糖的抗氧化能力进行了评价,通过正交试验设计方法对水提竹荪菌盖多糖的工艺条件进行了优化。结果表明,菌盖多糖的最佳提取条件为:提取温度85℃,料液比1∶25(g∶mL),浸提时间2.5 h。在此最佳工艺条件下,竹荪菌盖多糖得率为8.25%。菌盖多糖主要由葡萄糖、甘露糖和半乳糖组成,其中葡萄糖质量分数最高(58.19%)。菌盖多糖具有较强的还原能力和抑制羟基自由基能力。随着菌盖多糖质量浓度的升高,还原能力和抑制羟基自由基能力相应提高。但是,菌盖多糖对超氧阴离子的抑制能力较弱。Effects of different factors on extraction of polysaccharides from pileus of Dictyophora rubrovalvata were studied.The antioxidant activities of polysaccharides were evaluated.The extraction technology using water was optimized by orthogonal experiment.The optimum conditions were： extraction temperature 85℃,ratio of material to water 1∶25,extraction time 2.5h.Under the optimized experiment conditions,extractive yield was 8.25%.Polysaccharides were composed of glucose,mannose and galactose.Mass concentration of glucose content was the highest（58.19%）.Polysaccharides of pileus had high reducing power and scavenging hydroxyl radical activity.The reducing power and scavenging hydroxyl radical activity increased with the concentration of polysaccharides increased.However,scavenging superoxide anion radical of polysaccharides was weak.

关 键 词：红托竹荪 菌盖 多糖 正交试验 抗氧化能力 

分 类 号：TQ351.0[化学工程]