透明质酸在卡拉胶诱导的颞下颌关节滑膜炎模型中的作用  

Effects of Hyaluronan on Arrageenan-induced Synovitis in Rat TMJ

作　　者：汤涛 李明 朱宏飞[2] 

机构地区：[1]武汉市商业职工医院口腔科,武汉430020 [2]武汉大学口腔医学院

出　　处：《数理医药学杂志》2011年第3期292-296,共5页Journal of Mathematical Medicine

摘　　要：目的:探讨透明质酸及卡拉胶对颞下颌关节紊乱病程中细胞炎症反应和细胞增殖的影响。方法:在大鼠颞下颌关节中注射卡拉胶建立大鼠颞下颌关节滑膜炎模型,随后注射高分子量外源性透明质酸,观察卡拉胶和透明质酸注入前后关节滑膜的组织学变化,使用免疫组化法测定并评价透明质酸及卡拉胶对诱导型氧化氮合酶(iNOS),核转录因子κB(NF-κB)以及增殖细胞核抗原(PCNA)等免疫信号分子的调控作用以及对滑膜细胞增殖的影响。结果:卡拉胶可增加关节滑膜中iNOS、NF-κB等免疫阳性细胞及折叠样组织的数量;透明质酸则可减轻该作用。结论:卡拉胶可诱导增强炎症反应和关节滑膜细胞的增殖,而透明质酸可通过减少滑膜细胞产生的炎性介质从而减轻炎症反应。Objective：To elucidate and visualize the effects of carrageenan and hyaluronan on inflammation and cell proliferation in TMJ disorder.Methods：To histologically evaluate how inflammation and cell proliferation of the synovial membrane are affected by the exogenous administration of carrageenan and hyaluronan in the rat TMJ by investigating iNOS,NF-κB,and antiproliferating cell nuclear antigen（PCNA） immunoreactivity.Results：Carrageenan elevated iNOS,NF-κB,and cell proliferation resulting in an increase in synovial cell number and inflammation,which was relieved by hyaluronan.Conclusion：Carrageenan injection induced inflammation and cell proliferation especially in the synovial membrane and that hyaluronan relieved the inflammation by decreasing inflammatory molecules in the synovial membrane.

关 键 词：透明质酸 卡拉胶 颞下颌关节 诱导型氧化氮合酶 核转录因子ΚB 

分 类 号：R782.6[医药卫生—口腔医学]