微波消解ICP-MS法检测生物检材中汞元素  

Determination of Hg in Biological Samples by Inductively Coupled Plasma Mass Spectrometry

作　　者：马栋[1,2] 张丹[2,3] 卓先义[2] 刘伟[2] 沈保华[2] 沈敏[2] 

机构地区：[1]复旦大学上海医学院法医学系,上海200032 [2]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063 [3]苏州大学医学部法医学系,江苏苏州215123

出　　处：《法医学杂志》2011年第3期193-195,共3页Journal of Forensic Medicine

摘　　要：目的建立生物检材中汞的电感耦合等离子体质谱分析方法。方法采用微波消解法处理样品,以铟(115In)作内标,用电感耦合等离子体质谱仪对血液、尿液和头发中的汞含量进行分析。选择金与汞形成金汞齐,对金消除汞记忆效应的能力进行考察。结果方法检出限为0.01μg/L,准确度为97.0%～107.1%。检测中添加金质量浓度在10μg/L时,可消除检测中汞的记忆效应。结论建立的方法准确、快速、灵敏度高,适用于汞中毒案件的检测以及临床中汞中毒病人的诊断和监测。Objective To establish an inductively coupled plasma mass spectrometry（ICP-MS） method for determination of Hg in biological samples.Methods The samples were digested with microwave digestion instrument.ICP-MS was applied to detect Hg in blood,urine and hair specimens by using 115In as an internal marker.The ability of gold to eliminate the memory effect of mercury was investigated with the gold amalgamate produced by gold and mercury.Results The limits of detection were in the 0.01 μg/L,and the accuracy of the method ranged from 97.0% to 107.1%.The concentration of gold was 10 μg/L and the memory effect of mercury was resolved.Conclusion The method is accurate,rapid,sensitive and suitable for the cases of mercury poisoning and the clinical diagnosis and monitoring for patients with mercury poisoning.

关 键 词：法医毒理学 汞 电感耦合等离子体质谱法 微波消解 

分 类 号：R155.5[医药卫生—营养与食品卫生学]