重组巨大芽孢杆菌产青霉素G酰化酶发酵条件研究  

Studies on fermentation conditions for penicillin G acylase production with a recombinant Bacillus megaterium system

作　　者：范超[1] 黎继烈[1] 吴浩[2] 朱晓媛[1] 郝聚喜[1] 丁丽霞[1] 张蕾[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004 [2]深圳大学计算机与软件学院,广东深圳518060

出　　处：《中南林业科技大学学报》2011年第7期124-129,135,共7页Journal of Central South University of Forestry & Technology

基　　金：中南林业科技大学研究生科技创新基金项目(2009SX04)

摘　　要：对重组巨大芽孢杆菌产胞外青霉素G酰化酶的发酵条件进行优化。先用单因素实验确定最适碳源、氮源以及碳源浓度、氮源浓度、发酵时间和发酵温度的范围。采用Box-Behnken实验设计和响应面分析方法,对重组巨大芽孢杆菌产青霉素G酰化酶的发酵条件进行优化,得出其最佳发酵条件为:葡萄糖32 g/L、胰蛋白胨17 g/L、时间42.5 h、温度34℃,在此条件下青霉素G酰化酶的活力达到10 614±8 U/L,较优化前提高了16.83%。The fermentation conditions for extracellular penicillin G acylase producted by recombinant Bacillusmegaterium were optimized. At first, the optimal carbon, nitrogen and the range of carbon concentration, nitrogen concentration, fermentation temperature and fermentation time were determined by single factor experiments. The experiment used the Box-Behnken experimental design and response surface analytical methodology to optimize the fermentation conditions of production of penicillin G acylase of recombinant Bacillus megaterium. The optimum fermentation conditions were obtained： glucose 32 g/L, tryptone 17 g/L , time 42.5 hours, temperature 34 ℃. Under these conditions, the activity of penicillin G aeylase was 10 614±8 U/L, being increased by 16. 83% compared with 16. 83% before optimization.

关 键 词：重组巨大芽孢杆菌 青霉素G酰化酶 Box-Behnken实验设计 响应面法 发酵条件 

分 类 号：Q814[生物学—生物工程]