双菌种同步糖化共发酵造纸污泥生产乙醇的研究  

Papermaking sludge production of ethanol from paper mill sludge via two micro-organisms synchronous saccharification co-fermentation

作　　者：王明生[1,2] 何钢[1,2] 陈介南[1] 

机构地区：[1]中南林业科技大学生物环境科学与技术研究所 [2]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2011年第7期151-155,共5页Journal of Central South University of Forestry & Technology

基　　金：国家"十一五"科技支撑计划项目专题项目(2006BAD18B0503)

摘　　要：以APMP木浆造纸污泥为生物质原料,采用酿酒酵母与重组大肠杆菌KO11组合,同步糖化共发酵生产燃料乙醇,并对其工艺条件进行优化。结果表明:最适反应条件为温度38℃,底物浓度50 g/L,混合菌接种量为6%(体积分数),纤维素酶用量25 FPU/g底物,酿酒酵母与重组大肠杆菌KO11接种比例1?1(细胞干质量初始浓度分别为1.0 g/L和0.3 g/L)。在此工艺条件下发酵72 h,乙醇产率可达0.115 g乙醇/g底物,发酵效率达到理论值的42.31%。扫描电镜分析显示造纸污泥经同步糖化共发酵后,纤维含量减少,纸浆纤维被降解成小块碎片。Primary sludge from APMP pulp paper mill sludge was used as biomass material to produce fuel ethanol with Saccharomyces cerevisiae and E. coli KOll via simultaneous saccharifieation and co-fermentation（SSCF）. The SSCF process conditions were then optimized at a temperature of 38℃, with a substrate concentration of 50 g/L, a mixed bacteria inoeulums size of 6 % （ volume fraction）, and a cellulase dosage of 25 FPU/g, Saccharomyces cerevisiae and E. coli KOll inoculum ratio of 1 ： 1 （the initial concentration of cell dry mass were 1.0 g/L and 0. 3 g/L）. With these optimal technologcial conditions, the yield of ethanol reached 0. 115 g/g after a 72 hours incubation, the theoretical fermentation efficiency was 42. 31 % . Scanning electron microscopy （SEM） analysis shows that the paper- making sludge treated by SSCF, the fiber content decreased, pupl fibers were degraded into small fragments.

关 键 词：造纸污泥 纤维素酶 同步糖化共发酵 乙醇 

分 类 号：X17[环境科学与工程—环境科学]