抗纤复方I号对乙醛刺激的肝星状细胞的细胞外基质及细胞因子分泌的干预作用  

KangXian FuFang I inhibits acetaldehyde-stimulated extracellular matrix synthesis and cytokine secretion in cultured hepatic stellate cells

作　　者：林红[1] 黄玉红[1] 李异玲[1] 傅宝玉[1] 王炳元[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2011年第17期1826-1829,共4页World Chinese Journal of Digestology

摘　　要：目的:研究乙醛对肝星状细胞(HSC)细胞外基质和细胞因子分泌的影响及中药抗纤复方I号(KXI)的干预作用.方法:采用大鼠肝脏原位灌流消化法获得并原代及传代培养HSC,大鼠灌以KXI制备药物血清,以乙醛和药物血清作用于HSC,通过RT-PCR测定细胞内α1(I)型胶原mRNA的表达,以放射免疫法和ELISA法分别测定培养上清中透明质酸(HA)和转化生长因子-β1(TGF-β1)的含量.结果:100μmol/L乙醛刺激HSC后,HSC中α1(I)型胶原mRNA的表达、上清中HA(ng/L)及TGF-β1(ng/L)的含量增加(1.193±0.0344vs0.988±0.0208;1243.22±58.13vs602.33±194.06;2734.43±787.12vs559.92±97.63,均P<0.01).而100mL/L的药物血清则抑制α1(I)型胶原mRNA的表达、HA(ng/L)及TGF-β1(ng/L)的含量降低(0.973±0.0605vs1.193±0.0344;1032.667±77.65vs1243.22±58.13;759.62±205.34vs2734.43±787.12,均P<0.01).结论:乙醛能增加HSC中α1(I)型胶原的表达,并促进HSC分泌HA及TGF-β1,而KXI则起抑制作用.AIM： To investigate the effect of KangXian Fu-Fang I （KXI） on acetaldehyde-stimulated extracellular matrix synthesis and cytokine secretion in cultured hepatic stellate cells （HSCs）. METHODS： HSCs were isolated from rats and then primarily cultured and subcultured in vitro. Serum containing KXI was collected from normal rats intragastrically given the drug. HSCs were treated with acetaldehyde and the drugcontaining serum. The contents of hyaluronic acid （HA） and transforming growth factor β1 （TGF-β1） in the media and expression of type I collagen α1 mRNA in stimulated HSCs were measured by radioimmunoassay, ELISA and reverse transcription polymerase chain reaction（RT-PCR）, respectively. RESULTS： After HSCs were stimulated with acetaldehyde （100 μmol/L）, the expression level of type I collagen α1 mRNA （1.193 ± 0.0344 vs 0.988 ± 0.0208, P 0.05） in HSCs and secreted levels of HA （1243.22 ng/L ± 58.13 ng/L vs 602.33 ng/L ± 194.06 ng/L, P 0.05） and TGF-β1 （2734.43 ng/L ± 787.12 ng/L vs 559.92 ng/L ± 97.63 ng/L, P 0.01） in the media significantly increased. Drug-containing serum （10%） significantly inhibited acetaldehydestimulated expression of type I collagen α1 mRNA （0.973 ± 0.0605 vs 1.193 ± 0.0344, P 0.05） and secretion of HA （1032.667 ng/L ± 77.65 ng/ L vs 1243.22 ng/L ± 58.13 ng/L, P 0.05） and TGF-β1 （759.62 ng/L ± 205.34 ng/L vs 2734.43 ng/L ± 787.12 ng/L, P 0.01）. CONCLUSION： KXI could inhibit acetaldehydestimulated secretion of HA and TGF-β1 and expression of type I collagen α1 mRNA in cultured HSCs.

关 键 词：肝星状细胞 乙醛 抗纤复方I号 α1(I)型胶原 透明质酸 转化生长因子-Β1 

分 类 号：R575[医药卫生—消化系统]