肺癌组织Axin与DNMT1表达及其生物学意义的探讨  

Expressions and significances of Axin and DNMT1 in lung cancer tissues

作　　者：刘树立[1] 徐洪涛[1] 李庆昌[1] 杨连赫[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室中国医科大学附属第一医院病理科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2011年第9期676-679,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30801135)

摘　　要：目的:分析Axin和DNA甲基转移酶1(DNMT1)在肺癌组织中的表达和相关性,及其与肺鳞癌和腺癌临床病理因素的关系。方法:采用免疫组化方法检测Axin和DNMT1在100例肺癌组织中的表达情况。结果:Axin在肺鳞癌和腺癌组织中表达明显减少,其细胞质表达与肺癌的分化程度相关(P=0.044),核表达与肺癌的组织学类型(P=0.001)和TNM分期(P=0.016)相关。DNMT1在肺癌组织中的表达明显增加,并与肺鳞癌和腺癌的组织学类型(P=0.005)、分化程度(P=0.038)、TNM分期(P=0.031)和淋巴结转移(P=0.032)显著相关。Axin在肺癌组织中的低表达与DNMT1高表达显著负相关(r=-0.222,P=0.027),而在肺腺癌组织中Axin的核表达也与DN-MT1的表达显著负相关,r=-0.308,P=0.026。结论:Axin在肺鳞癌和腺癌组织中的低表达与DNMT1的高表达显著相关。DNMT1的表达可以作为肺癌恶性程度和进展情况的指标之一。OBJECTIVE： To examine the expressions and correlations of Axin and DNA methyltransferase 1 （DNMT1） in lung cancers, and their relationships with the clinicopathological parame ters of lung squanous cell carcinomas and adenocarcinomas. METHODS： The expression patterns and levels of Axin and DNMT1 were examined using immunohistochemistry in 100 lung cancer tissue samples. RESULTS： The expression of Axin was reduced significantly in lung cancers. The cytoplasmic expression of Axin was associated with the differentiation （P=0. 044） of lung cancers; the nuclear expression of Axin was associated with the histological type （P= 0. 001） and TNM stage （P=0. 016） of lung cancers. The expression of DNMT1 was enhanced significantly, and associated with the histological type （P = 0. 005）, differentiation （P = 0. 038）, TNM stage （P=0. 031） and lymph node metastasis （P=0. 032） of lung cancer samples. The reduced expression of Axin was significantly correlated with the high expression of DNMT1 （r=-0. 222, P=0. 027）. Fur thermore, the nuclear expression of Axin was also negatively correlated with the expression of DNMT1 in lung adenocareinoma tissues （r=-0. 308, P=0. 026）. CONCLUSIONS： The reduced expression of Axin is correlated with the high expression of DNMT1 in lung cancers. The expression level of DNMT1 can be a mark to indicate the grade malignancy and progression of lung cancers.

关 键 词：肺肿瘤/病理学 AXIN DNA甲基化 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]