石英的人类致癌性在DNA分子水平的证据  

Evidence on the carcinogenicity of silica at DNA level in human

作　　者：刘秉慈[1] 关然[1] 周培宏[1] 缪庆[1] 王海华[1] 尤宝荣[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050

出　　处：《卫生研究》1999年第5期257-258,共2页Journal of Hygiene Research

基　　金：国家自然科学基金;卫生部科研基金

摘　　要：收集３６例石英粉尘作业工人肺癌（石英肺癌）的组织石蜡包埋物，提取其 Ｄ Ｎ Ａ，对 ｐ５３基因和 Ｋｒａｓ 基因进行了 Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ、 Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ及 Ｄ Ｎ Ａ 测序分析，发现具有完全有别于普通肺癌的特异基因突变谱。小细胞肺癌的 ｐ５３基因突变多发于第８外显子，腺癌的 ｐ５３基因突变率高。石英肺癌的 Ｋｒａｓ 基因突变未见一例发生于普通肺癌突变热点区第１２密码子上，相反发现多处非热点区突变，突变类型以 Ｇ→ Ｃ 突变为主。The alteration of p53 and K\|ras gene in 36 lung cancers of workers occupationally exposed to silica(LCWS) was studied.DNA was extracted from paraffin\|embedded tissues and amplified by PCR.PCR\|SSCP analysis on exons 5,7 and 8 of p53 gene revealed that 43 8% of mutations were clustered in exon 8 of p53 gene.In ordinary lung cancer,the mutation rate of exon 8 was fluctuated around 20%.The prevalence of mutations was the highest (70%) in small cell lung cancer(SCLC) and the lowest in adenocarcinoma(33%).In our study the mutation rate was high in adenocarcinoma(53 9%) and low in SCLC (30 8%).The K\|ras gene of LCWS also revealed a different mutation pattern.Unexpectedly,no mutation was found at codon 12 in 36 LCWS by PCR\|RFLP,which is the predominant mutation in K\|ras gene in ordinary lung cancer.The point mutation study by DNA sequencing confirmed the absence of codon 12 mutation in K\|ras gene in LCWS.The mutations observed in this study were frequently distributed in codons 13 and 15,principally a G\|C transversion,instead of G\|T transversion in ordinary lung cancer. $$$$

关 键 词：矽肺 肺癌 基因突变 

分 类 号：R734.202[医药卫生—肿瘤] R135.2[医药卫生—临床医学]