十二烷基磺酸钠-盐酸维拉帕米-联吡啶钌体系电化学发光及其应用  

Electrochemiluminescence of Sodium Dodecyl Sulfate-Verapamil Hydrochloride-Ru(bpy)_3^(2+) System and Its Application

作　　者：李利军[1] 高文燕[2] 蔡卓[2] 胡大春[1] 李彦青[1] 赖映标[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]广西大学化学化工学院,南宁530004

出　　处：《分析化学》2011年第7期1033-1037,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然基金(No.20665001);桂科自(No.0832062)资助项目

摘　　要：在十二烷基磺酸钠(SDS)中,考察了盐酸维拉帕米-Ru(bpy)32+体系在金电极上的电化学及其发光行为。结果表明:SDS对体系的电化学反应和电化学发光强度具有显著的增敏作用。据此,建立了一种高效、简便的测定盐酸维拉帕米的电化学发光新方法。在最佳实验条件下,盐酸维拉帕米浓度在1.0×10-4～1.0×10-7mol/L范围内与相对发光强度呈线性关系,检出限(S/N=3)为5.2×10-10 mol/L,连续平行测定1.0×10-5 mol/L盐酸维拉帕米5次,发光强度的RSD为3.1%。对样品进行回收实验,回收率在98.7%～106.5%之间,RSD为3.0%(n=5)。本方法具有较高的选择性和灵敏度,样品处理简单,用于实际样品的测定,结果满意。When [SDS] was 0.3125 mmol/L,the electrochemical and ECL behaviours of Ru（bpy）32＋-verapamil hydrochloride（yswlpm） system at the Au electrode were studied.An enhancement electrochemical activity and ECL response were exhibited in the presence of SDS.Base on this,ECL method was established for the determination of verapamil hydrochloride.Under the optimum experimental conditions,the ECL intensities had good linear relationship with the concentration of the verapamil hydrochloride in the range of 1.0×10-4-1.0×10-7 mol/L（r=0.9958）.The detection limit was 5.2×10-10 mol/L.The RSD for 5 times determination of 1.0×10-5 mol/L verapamil hydrochloride was 3.1%.The results of recovery were between 98.7%-106.5%,the RSD for 5 times was 3.0%.This proposed method showed high selectivity and sensitivity,and the sensitivity,and the disposal method of the sample was simple.

关 键 词：十二烷基磺酸钠 联吡啶钌 电化学发光 盐酸维拉帕米 

分 类 号：R914[医药卫生—药物化学]