大孔吸附树脂分离纯化花椒总黄酮的研究  

Study on separation and purification of flavonoids in Zanthoxylum bungeanum by macroporous adsorption resin

作　　者：吴亮亮[1,2] 石雪萍[1] 张卫明[1] 

机构地区：[1]南京野生植物综合利用研究院,江苏南京210042 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品工业科技》2011年第7期260-262,266,共4页Science and Technology of Food Industry

基　　金：国家"十一五"科技支撑计划重点课题(2006BAD06B02)

摘　　要：通过比较了6种大孔吸附树脂D4006、NKA-9、X-5、HPD450、HPD600和AB-8对花椒总黄酮的吸附及脱附性能,筛选出最佳树脂进行动态实验研究,得到优化的纯化条件。结果表明:AB-8型大孔树脂纯化效果最好,其最佳工艺为:上样浓度3.0～4.0mg/mL,上柱pH4.5左右,上柱速度2.5mL/min,洗脱剂最适宜体积分数与洗脱剂用量分别为70%和3BV(柱体积bed volume)。经AB-8型大孔吸附树脂纯化后,花椒黄酮的纯度由51.46%提高到90.25%,总黄酮回收率81.65%。对经AB-8大孔树脂纯化后的花椒黄酮进行高效液相色谱(HPLC)分析发现,黄酮纯度显著提高,其中芦丁含量较高。Adsorption and desorption characteristics of macroporous adsorption resins D4006, NKA-9, X-5, HPD450,HPD600 and AB-8 toward flavones of Zanthoxylum bungeanum were investigated.The best macroporous adsorption resin was chosen to carry out dynamic adsorption experiments, while the purification process was acquired.The results showed that the AB-8 macroporous resin gave the best effect of separation with concent ratio 3.0-4.0mg/mL,the feed solution pH 4.5 and the sampling rate 2.5mL/min, respectively, and the concent ratio of elution was 70% alcohol, with eluant volume 3BV （ bed volume）. By this technology the flavonoid content was increased from 51.46% of the crude extract to 90.25% of the purified product and the yield was 81.65%. High performance liquid chromatography （HPLC） analysis result showed that the purity of obtained flavonoids was significantly increased,and the rutin content was higher than other flavonoids.

关 键 词：花椒 黄酮 大孔吸附树脂 纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]