二十二碳六烯酸对老年大鼠脑组织抗氧化和脂肪酸的影响  

Effect of DHA on Antioxidation and Fatty Acids in Aged Rat Brain

作　　者：蒋利和[1,2] 马博[2,3] 谢正轶[4] 杨志荣[2] 

机构地区：[1]广西大学科技处,广西南宁530004 [2]四川大学生命科学学院,四川成都610064 [3]百色学院化学与生命科学系,广西百色533000 [4]广西医科大学人体解剖学教研室,广西南宁530021

出　　处：《食品科学》2011年第13期284-288,共5页Food Science

基　　金：2005四川省科技条件平台项目(川财教:2005172);广西大学博士启动项目(X090015)

摘　　要：目的:研究二十二碳六烯酸(DHA)改善老年大鼠抗氧化的可能机制。方法:动物分为青年组(7月龄青年雄性大鼠)、老年组(20月龄雄性大鼠)、DHA低剂量组(灌喂20月龄雄性大鼠DHA 180mg/(kg.d)42d)、DHA高剂量组(灌喂20月龄雄性大鼠360mg/(kg.d)42d),处死大鼠后测定脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活力、丙二醛(MDA)含量,然后用气相色谱-质谱联用分析法分析脑脂肪酸组成及含量,最后用薄层层析和磷钼酸法分析磷脂构成。结果:与老年大鼠相比,灌喂DHA的各组大鼠的脑中SOD、GSH-Px、CAT活性显著提高,同时灌喂DHA减少MDA的含量;对脑脂肪酸和磷脂的分析,表明灌喂DHA显著提高脑DHA含量,增加总n-3脂肪酸/总n-6脂肪酸和DHA/AA(二十碳四烯酸)的比率,显著降低AA含量,还能显著提高磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇、磷脂酰丝氨酸及神经鞘磷脂的含量。结论:DHA显著提高自然衰老大鼠的脑组织抗氧化能力,其机制可能与改变脑脂肪酸组成有关。Objective：To explore the antioxidant mechanisms of DHA in the brain of aged rats.Methods：Wistar male rats were randomly divided into four groups including 7-month-old adult group,20-month-old aged group,and low-dose and high-dose DHA-treated 20-month-old aged groups at the respective doses of 180 mg/（kg·d） and 360 mg/（kg·d）（administered by gavage for 42 consecutive days）.At the end of the administration period,all rats were sacrificed and the activities of superoxide dismutase（SOD）,gtutathione peroxidase（GSH-Px） and catalase（CAT） and the level of malondialdehyde（MDA） were assayed in brain.In addition,the fatty acid profile in brain was analyzed by gas chromatography-mass spectrometry,and the phospholipid composition was also analyzed by thin layer chromatography and phosphomolybdate methods.Results：Compared with the aged group,the activities of SOD,GSH-Px and CAT in the brain of the DHA-treated groups were significantly increased and MDA content was significantly decreased.Fatty acid analysis showed that DHA-treated rats exhibited an obvious increase in the ratio of total n-3 fatty acids to total n-6 fatty acids and the ratio of DHA to arachidonic acid（AA） and an obvious reduction in AA level.It was also revealed that DHA could improve the levels of phosphatidylcholine,phosphatidylethanolamine,phosphatidylinositol,phosphatidylserine and sphingomgelins in the brain of rats.Conclusion：DHA can obviously improve antioxidant capacity in aged rats and its antioxidant mechanism may be due to DHA-induced change of fatty acid profile in the brain.

关 键 词：二十二碳六烯酸 抗氧化 脂肪酸 

分 类 号：Q943.5[生物学—植物学]