可跨膜SOD对肝癌及正常肝细胞增殖的作用  

Effects of transductable SOD on the proliferation of hepatocarcinoma and normal hepatic cell lines

作　　者：刘树滔[1] 陆重益[1] 赵花琴[1] 张晨[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002

出　　处：《福州大学学报（自然科学版）》2011年第3期460-463,共4页Journal of Fuzhou University(Natural Science Edition)

摘　　要：可跨膜融合蛋白PTD-SOD与肝癌细胞和正常肝细胞共培养,使细胞内SOD活力分别增加了52%和23%,提高胞内总抗氧化能力(T-AOC)水平达100%,同时降低ROS水平.实验结果显示,肝癌细胞增殖受到显著抑制,最高抑制率达91.91%,对正常肝细胞抑制率较小,为45.50%.Incubated with PTD -SOD （PTD, protein transduction domain; SOD, superoxide dismutase） fused protein, the intracellular SOD activity of HepG2 and L -02 cell lines increased obviously, by 52% and 23% respectively. IntraceUular total antioxidant capacity（T- AOC） was up to 100% and ROS level was decreased remarkably as well. Dates showed that the proliferation of HepG2 cell was significantly suppressed, up to 91.91% ; while that of L -02 cell was only to 45.50%.

关 键 词：PTD—SOD 肝癌 抗氧化 自由基 细胞 

分 类 号：Q2[生物学—细胞生物学]