山东食管癌高发区脱氧雪腐烯醇和硒元素含量检测研究  

Determination of Deoxynivalenol and Selenium in High Incidence Areas with Esophageal Cancer in Shandong

作　　者：侯海峰[1] 陈龙明 邱静[3] 李金平[1] 董方[1] 李群伟[1] 

机构地区：[1]泰山医学院,山东泰安271000 [2]莱钢集团,山东莱芜271104 [3]泰安市疾病预防控制中心,山东泰安271000

出　　处：《中国肿瘤》2011年第6期406-408,共3页China Cancer

基　　金：山东省自然科学基金项目(Y2008C59);山东省卫生厅资助项目(2005HW142);山东省教育厅资助项目(J07E06)

摘　　要：[目的]探讨山东省农村居民粮食中脱氧雪腐镰刀菌烯醇(DON)和硒元素(Se)的含量与食管癌高发的关系。[方法]粮食样品中DON毒素含量的检测采用酶联免疫吸附法(ELISA);硒元素的检测采用2,3-二氨基萘(DAN)荧光分光光度法。[结果]东平、肥城两个食管癌高发区粮食DON含量为70.1～302.8μg/kg,对照组(泰山区、长清区)粮食DON检测结果为低于27.3μg/kg,食管癌高发区与对照区粮食DON含量差异有统计学意义(P<0.05);东平、肥城两个食管癌高发区粮食Se含量均数分别为76.5μg/kg、85.6μg/kg,对照组泰山区、长清区粮食Se含量均数分别为83.1μg/kg、90.7μg/kg,对照地区高于食管癌高发区(P<0.05);东平、肥城、泰山区、长清区居民头发Se含量均数分别为0.926mg/kg、1.015mg/kg、0.937mg/kg、0.941mg/kg,高发区组和对照组间差异无统计学意义(P>0.05)。[结论]食管癌发病可能与DON有关,Se的缺乏可能不是食管癌发病的主要病因。[Purpose] To investigate relationship between high incidence of esophageal cancer and content of deoxynivalenol（DON）,selenium（Se） in grains in rural residents in Shandong Province.[Methods] DON was quantified by enzyme linked immunosorbent assay（ELISA）.Quantitative determination of Se was performed with 2,3-DAN fluorescence spectrophotometry.[Results] The concentration of DON in grains in high incidence area,of esophageal cancer Dongping and Feicheng was 70.1～302.8μg/kg respectively.The concentration in control areas,Taishan and Changqing was less than 27.3μg/kg.There was significant difference between the two groups（P0.05）.The concentration of Se in grains in the two esophageal cancer high incidence Dongping and Feicheng areas was 76.5μg/kg and 85.6μg/kg,while it was 83.1μg/kg and 90.7μg/kg in control areas respectively,with significant difference（P0.05）.Content of Se in hairs in Dongping,Feicheng,Taishan and Changqing was 0.926mg/kg,1.015mg/kg,0.937mg/kg and 0.941mg/kg,respectively,without significant difference between high incidence areas group and control areas group（P0.05）.[Conclusion] DON might relate to carcinogenesis of esophageal cancer.Lack of Se might not be the main risk factor for carcinogenesis with esophageal cancer.

关 键 词：脱氧雪腐镰刀菌烯醇 硒 食管癌 山东 危险因素 

分 类 号：R735.1[医药卫生—肿瘤] R73-31[医药卫生—临床医学]